İnci Başak MÜŞTAK, Hamit Kaan MÜŞTAK, Seyyide SARIÇAM, Tuğçe ÜSTÜN, Asena Esra ERDEM

Köpek Deri Enfeksiyonlarında Staphylococcus pseudintermedius Sıklığı ve Elde Edilen İzolatların Antibiyotik Direnç Profilleri

Bu çalışmada, deri enfeksiyonu olan 77 köpekten 61 Staphylococcus pseudintermedius suşu izole edilmiştir. Yaygın olarak kullanılan antibiyotiklere karşı antimikrobiyal direnç, disk difüzyon yöntemi ile değerlendirildi. Penisilin ve metisilin direncinden sorumlu sırasıyla blaZ ve mecA genlerinin varlığı PCR ile araştırıldı. S. pseudintermedius izolatları tetrasiklin (% 47.5) ve penisiline (% 40.9) yüksek direnç gösterirken, tüm izolatlar amikasine (% 100) duyarlıydı. 18 metisiline dirençli S. pseudintermedius (MRSP) suşunun tümü mecA geni için pozitif iken blaZ genine sahip 25 S. pseudintermedius suşu izolatı da fenotipik olarak penisiline dirençliydi. 18 MRSP izolatından 14 S. pseudintermedius suşu, üç veya daha fazla antibiyotiğe dirençliydi, bu da bu suşların çoklu ilaç direnci (MDR) gösterdiğini ortaya koydu. Bu rapor, S. pseudintermedius'un köpek deri enfeksiyonlarından en sık izole edilen bakteri olduğuna dair önceki bulguları doğrulamaktadır. Bu çalışmada test edilen tüm S. pseudintermedius suşları amikasine duyarlı olduğundan, amikasinin S. pseudintermedius enfeksiyonlarının tedavisinde yararlı bir ajan olduğu sonucuna varılabilir.

Anahtar Kelimeler:

köpek, metisilin direnci, staphylococcus pseudintermedius

Frequency of Staphylococcus pseudintermedius in Canine Skin Infections and Antibiotic Resistance Profiles of the Recovered Isolates

In this study, 61 Staphylococcus pseudintermedius strains were isolated from 77 dogs with skin infections. Antimicrobial resistance to commonly used antibiotics was evaluated by disc diffusion method. The presence of the blaZ and mecA genes responsible respectively for penicillin and methicillin resistance were investigated by PCR. The S. pseudintermedius isolates were highly resistant to tetracycline (47.5%) and penicillin (40.9%) while all isolates were susceptible to amikacin (100%). All 18 methicillin-resistant S. pseudintermedius (MRSP) strains were positive for the mecA gene while the 25 S. pseudintermedius strain isolates with the blaZ gene were also resistant to penicillin phenotypically. Of the 18 MRSP isolates, 14 S. pseudintermedius strains were resistant to three or more antibiotics, indicating that these strains showed multiple drug resistance (MDR). This report confirms previous findings that S. pseudintermedius is the most frequently isolated bacteria from dog skin infections. It can also be concluded that amikacin is a useful agent for treating S. pseudintermedius infections since all the S. pseudintermedius strains tested in this study were susceptible to amikacin.

Keywords:

dogs, methicillin resistance, Staphylococcus pseudintermedius,

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