Hüseyin AKŞİT, Samed ŞİMŞEK, Volkan ÖZDOKUR, Ekrem KÖKSAL

Arbutin’in yeni kaynakları: Onobrychis nitida and Onobrychis galegifolia

Arbutin (4-Hidroksifenil-β-D-glukopiranozit), hidrokinonun mono glikozidik formudur ve cilt beyazlatma ve çil giderme özellikleri nedeniyle kozmetiklerde uzun süredir yaygın olarak kullanılmaktadır. Bu çalışmada; arbutin flash kromatografi kullanılarak Onobrychis galegifolia'nın metanol ekstraktından izole edilmiştir. Arbutin yapısı, 1H ve 13C -NMR spektroskopisi ve ayrıca 2D NMR teknikleri ile aydınlatıldı. Ek olarak, O. galegifolia ve O. nitida türlerinde bulunan arbutinin kantitatif analizi HPLC-DAD kullanılarak yapıldı. Sonuçlara göre, O. galegifolia ve O. nitida'nın metanol ekstraktlarının arbutin içeriği sırasıyla 212.02 ve 196.46 mg/g ekstrakt olarak bulundu. Ayrıca, O. galegifolia ve O. nitida'nın soğuk su ekstraktlarının arbutin içeriği 128.2 ve 99.40 mg/g ekstrakt olarak bulundu. Kuru ağırlıklardaki arbutin miktarı, yaklaşık% 5.66 ila% 6.86 arasında hesaplandı.

Anahtar Kelimeler:

Onobrychis nitida, Arbutin

New sources of arbutin: Onobrychis nitida and Onobrychis galegifolia

Arbutin (4-Hydroxyphenyl-β-D-glucopyranoside) is a mono glycosidic form of hydroquinone and has been widely used in cosmetics for a long time due to skin whitening and freckle removing properties. In this study, arbutin was isolated from the methanol extract of Onobrychis galegifolia using flash chromatography. The structure of arbutin was elucidated by 1H and 13C -NMR spectroscopy and as well as 2D NMR techniques. In addition, quantitative analysis of arbutin found in O. galegifolia and O. nitida species was done using HPLC-DAD. According to the results, the arbutin content of methanol extracts of O. galegifolia and O. nitida was found 212.02 and 196.46 mg/g extract respectively. In addition, the arbutin content of cold-water extracts of O. galegifolia and O. nitida was found 128.2 and 99.40 mg/g extract. The amount of arbutin in dry weights was calculated for about 5.66% to 6.86%.

Keywords:

Arbutin, Onobrychis nitida,

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