Bilal Akyüz, Korhan Arslan, Esma Gamze İlgar

Türkiye’de Yetiştirilen Anadolu Mandalarında Butirofilin (BTN1A1) Gen Polimorfizminin HaeIII Restriksiyon Enzimi ile Araştırılması

Butirofilin süt yağının salgılanmasında önemli rolü olan bir transmembran glikoproteinidir. Bu araştırmada, Türkiye’de yetiştirilen Anadolu mandalarında butirofilin (BTN1A1) geninin genotip ve allel frekanslarının belirlenmesi amaçlanmıştır. Bu çalışmada toplam olarak 120 manda polimeraz zincir reaksiyonu-restriksiyon parçacık uzunluk polimorfizmi (PCR-RFLP) metodu ile genotiplendirilmiştir. Çalışmada yapılan PCR işlemi sonunda elde edilen 501 bç’lik amplikonlar elde edilmiştir. Elde edilen PCR ürünlerinin HaeIII restiriksiyon enzimi ile kesilmeleri sonucunda incelenen örneklerde sadece A alleli ve AA genotipinin bulunduğu görülmüştür. Bu çalışma Anadolu mandalarında BTN1A1 gen polimorfizminin araştırıldığı ilk çalışmadır. Çalışma sonunda, Anadolu mandalarında BTN1A1 geninde polimorfizm bulunamamıştır.

Anahtar Kelimeler:

Anadolu mandası, butirofilin geni, genetik polimorfizm, RFLP

Investigation of Butyrophilin (BTN1A1) Gene Polymorphism by Using HaeIII Restriction Enzyme in Anatolian Buffaloes Reared in Turkey

Butyrophilin is a transmembrane glycoprotein and plays a critical role in secretion of milk lipid. The aim of this study was to identify genotype and allele frequencies of butyrophilin (BTN1A1) gene in Anatolian buffaloes in Turkey. A total of 120 buffalos were genotyped by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in this study. As a result of PCR process, 501 bp amplicons were obtained from the Anatolian buffaloes examined in this study. After digestion of the PCR products with HaeIII restriction enzyme, only A allele and AA genotype were found. This study provided first information on the polymorphism on BTN1A1 gene in Anatolian buffaloes. In conclusion, there was no polymorphism found in the BTN1A1 gene in Anatolian buffaloes.

Keywords:

Anatolian buffalo, butyrophilin gene, genetic polymorphism, RFLP,

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