Sodyum Laktat ve Timol’ün Aynalı Sazan Balığı’ndan (Cyprinus carpio L.) Yapılan Köftelerin Bazı Mikrobiyolojik ve Duyusal Nitelikleri Üzerine Etkisi*

Bu çalışma, aynalı sazan (Cyprinus carpio L.) balığı’ndan yapılan köftelerin bazı mikrobiyolojik, kimyasal ve duyusal özellikleri üzerine timol ve sodyum laktat’ın tek başlarına ve kombine halde kullanımlarının etkilerini araştırmak amacıyla yapıldı. Hazırlanan köfte örnekleri altı gruba (kontrol, %1 sodyum laktat, %0.1 timol, %0.25 timol, %1 sodyum laktat + %0.1 timol ve %1 sodyum laktat + %0.25 timol) ayrıldı ve buzdolabı şartlarında (4±1°C) muhafazaya alınarak 0., 4., 6., 8. ve 10. günlerde mikrobiyolojik (psikrofilik aerobik mikroorganizma, koliform ve maya-küf), kimyasal (pH) ve duyusal (lezzet, koku, renk) nitelikleri bakımından incelendi. Mikrobiyolojik analiz sonuçları, %1 sodyum laktat ile %1 sodyum laktat + %0.25 timol ilave edilmiş köfte örneklerinin diğer gruplara göre daha uzun muhafaza süresine sahip olduklarını (p<0.05) gösterdi. Duyusal değerlendirme sonuçları lezzet açısından gruplar arasında farklılıklar olduğunu (p>0.05), artan timol konsantrasyonuna bağlı olarak bazı grupların kabul edilemez bir aromaya sahip olduklarını gösterdi. Sonuç olarak, balık köftelerine ilave edilen timol’ün mikrobiyel ve muhafaza süresi açısından önemli sayılabilecek bir etki göstermediği, ancak duyusal açıdan ise ürünün nitelikleri üzerine olumsuz etkiye sahip olduğu ortaya konuldu

Effect of Sodium Lactate and Thymol on Some Microbiological, Chemical and Sensory Attributes of Fish Patty Made from Mirror Carp Meat (Cyprinus carpio L.)

The present study was carried out to investigate the effects of thymol and sodium lactate, alone or in combination, on some microbiological, chemical and sensory attributes of fish patty made from common carp meat. Fish patty samples were separated into 6 groups (control, 1% sodium lactate, 0.1% thymol, 0.25% thymol, 1% sodium lactate + 0.1% thymol and, 1% sodium lactate + 0.25% thymol) and stored at 4°C and analyzed for microbiological (psychrophilic aerobic microorganisms, coliforms, yeast and mold), chemical (pH) and sensory (taste, odor and color) attributes on days 0, 4, 6, 8 and 10. Results of the microbiological analysis indicated that the fish ball samples treated with 1% sodium lactate and 1% sodium lactate + 0.25% thymol have longer shelf-life compared to the other groups (p<0.05). Results of the sensory evaluations showed that there were differences between the groups in point of taste (p<0.05), and some groups have an unacceptable aroma depending on the increase in the thymol concentration. As a result, it was concluded that addition of thymol into the fish patty did not show significant effect on the microbiological quality and shelf-life of the product; however it has a negative effect on the sensory attributes of the product.

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  • 1. Al-Harbi AH, Uddin MN. Aerobic bacterial flora of common carp (Cyprinus carpio L.) cultured in Earthen Ponds in Saudi Arabia. Journal of Applied Aquaculture 2008; 20: 108-19.
  • 2. Akkuş Ö, Varlık C, Erkan N, Mol S. Çiğ ve haşlanmış balık etinden yapılmış köftelerin bazı kalite parametrelerinin incelenmesi. Turk J Vet Anim Sci 2004; 28: 79-85.
  • 3. AOAC. Association Official Analytical Chemists. Official methods of analysis of the association of official analytical chemist. 15th Edition, Washington DC, 1990.
  • 4. Bagamboula, CF, Uyttendaele M, Debevere J. Inhibitory effect of thyme and basil essential oils, carvacrol, thymol, estragol, linalool and p-cymene towards Shigella sonnei and S. flexneri. Food Microbiol 2004; 21: 33–42.
  • 5. Bajpai VK, Baek K-H, Kang SC. Control of Salmonella in foods by using essential oils: A review. Food Res. Int 2012; 45: 722–34.
  • 6. Burt S. Essential oils: their antibacterial properties and potential applications in foods- a review. Int J Food Microbiol 2004; 94: 223-53.
  • 7. Cahill MM. Bacterial flora of fishes: A review. Microbial Ecology 1990; 19: 21-41.
  • 8. Çetin B, Bostan K. Hazır köftelerin mikrobiyolojik kalitesi ve raf ömrü üzerine sodyum laktatın etkisi. Turk J Vet Anim Sci 2002; 26: 843-48.
  • 9. Ersoy B, Yılmaz AB. Karabalık ( Clarias gariepinus BURCHELL,1822) köftesinin dondurularak muhafazası. Turk J Vet Anim Sci 2003; 27: 827-32.
  • 10. Göktan D. Gıdaların mikrobiyal ekolojisi, Et Mikrobiyolojisi. Ege Üniversitesi Basımevi, İzmir, 1990.
  • 11. Gutierrez J, Barry-Ryan C, Bourke P. The antimicrobial efficacy of plant essential oil combinations and interactions with food ingredients. Int J Food Microbiol 2008; 124: 91-97.
  • 12. Halkman AK. Merck Gıda Mikrobiyolojisi Uygulamaları. Ankara, 2005.
  • 13. Harrigan WF. Laboratory methods in food microbiology. 3rd Edition, London, 1998.
  • 14. ICMSF. International Commission on Microbiological Specification for Foods. Microorganisms in Foods. 2. Sampling for microbiological analyses: principles and specific applications. 2nd Edition, Toronto, 1986.
  • 15. Juven BJ, Kanner J, Schved F, Weisslowicz H. Factors that interact with the antibacterial action of thyme essential oil and its active constituents. J Appl Bacteriol 1994; 76: 626–31.
  • 16. Lambert RJW, Skandamis PN, Coote PJ, Nychas GJE. A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol. J Appl Microbiol 2001; 91: 453–62.
  • 17. Lv FEI, Liang H, Yuan Q, Li C. In vitro antimicrobial effects and mechanism of action of selected plant essential oil combinations against four food-related microorganisms. Food Res Int 2011; 44: 3057–64.
  • 18. Mahaffey KR. Fish and shellfish as a dietary sources of methylmercury and the ω-3 fatty acids, eicosahexaenoic acid and docosahexaenoic acid: risk and benefits. Environ Res 2004; 95 (3): 414-28.
  • 19. Mahmoud BSM, Yamazaki K, Miyashita K et al. Bacterial microflora of carp (Cyprinus carpio) and its shelf-life extension by essential oil compounds. Food Microbiol 2004; 21: 657- 666.
  • 20. Mani-López E, Garcia HS, López-Malo A. Organic acids as antimicrobials to control Salmonella in meat and poultry products. Food Res Int 2012; 45: 713-21.
  • 21. Metin S, Erkan N, Varlık C. The application of Hypoxanthine Activity as a Quality indicator of cold stored fish burgers. Turk J Vet Anim Sci 2002; 26: 363-67.
  • 22. Öksüztepe G, Çoban ÖE, Güran HŞ. Sodtum laktat ilavesinin taze gökkuşağı alabalığından (Oncorhynchus mykiss W) yapılan köftelere etkisi. Kafkas Univ Vet Fak Derg 2010; 16 (Supply A): 65-72.
  • 23. Özpolat E, Çoban ÖE. Kara balık (Capoeta trutta, heckel, 1843) ve Sarı balığın (Capoeta umbla, Heckel, 1843) köfte olarak değerlendirilmesi ve kalite kriterleri üzerine farklı muhafaza sıcaklıklarının etkisi. Su Ürünleri Dergisi 2012; 29 (3): 127-31.
  • 24. Patır B, Inanlı AG, Oksuztepe GA, Dincoglu AH, Ilhak OI. Shelf-life, sensory attributes and microbiological and chemical characteristics of processed mirror carp (Cyprinus carpio L) fillets. Journal of Animal and Veterinary Advances 2009; 8: 1101-09.
  • 25. Schirmer BC, Langsrud S. Evaluation of natural antimicrobials on typical meat spoilage bacteria ın vitro and in vacuum-packed pork meat. J Food Sci 2010; 75: M98-M102.
  • 26. Tajkarimi MM, Ibrahim SA, Cliver DO. Antimicrobial herb and spice compounds in food. Food Control 2010; 21: 1199–18.
  • 27. Tiwari BK, Valdramidis VP, O’donnell CP, Muthukumarappan K, Bourke P, Cullen PJ. Application of natural antimicrobials for food preservation. J Agric Food Chem 2009; 57: 5987–00.
  • 28. Valverde IM, Periago MJ, Santaella M. The content and nutritional significance of minerals on fish flesh in the presence and absence of bone. Food Chem 2000; 71: 503-09.
  • 29. USDA / Food Safety and Inspection Service. Food additives for use in meat and poultry products: Sodium diacetate, sodium acetate, sodium lactate and potassium lactate. In: Rules and regulations. Fed. Reg. 65(3), 3121–3123. 9CFR Part 424, (Docket No. 99-028DF). 2000.
  • 30. Xu J, Zhou F, Ji BP, Pei RS, Xu N. The antimicrobial mechanism of carvacrol and thymol against Escherichia coli. Lett Appl Microbiol 2008; 47: 174–79.
Erciyes Üniversitesi Veteriner Fakültesi Dergisi-Cover
  • ISSN: 1304-7280
  • Yayın Aralığı: Yılda 3 Sayı
  • Başlangıç: 2004
  • Yayıncı: Erciyes Üniv. Veteriner Fak.