Sıçanlarda Emamektin Benzoat Kaynaklı Oksidatif Hasara Karşı Diosminin Koruyucu Etkilerinin Araştırılması

Bu çalışma ile sıçanlarda emamektin benzoat (EB) maruziyetine karşı diosminin (DİO) etkilerinin araştırılması amaçlandı. Çalışmada her grupta 10 tane olacak şekilde toplam 60 adet 6-8 haftalık 180-250 g ağırlığında Wistar Albi- no ırkı erkek sıçan kullanıldı. Gruplar sırasıyla kontrol, EB (10 mg kg-1), DİO (50 mg kg-1), DİO (100 mg kg-1), EB (10 mg kg-1) + DİO (50 mg kg-1), EB (10 mg kg-1) + DİO (100 mg kg-1) olarak belirlenerek belirtilen miktarlarda 21 gün boyunca gavajla uygulama yapıldı. Deneme süresinin sonunda anestezi altında kalbe punksiyon yapılarak heparinize ve antikoagulansız tüplere kan örneği alındı. Servikal dislokasyon sonrası sıçanların karaciğer, böbrek, beyin, testis, kalp ve akciğer dokuları çıkarıldı. Serumda bazı biyokimyasal parametreler (aspartat aminotransferaz (AST), alanin aminotransferaz (ALT), laktat dehidrogenaz (LDH), alkalin fosfataz (ALP), trigliserit, kolesterol, total protein, albümin, ürik asit, kan üre azotu (BUN) ve kreatinin) ölçülürken doku örneklerinde ise oksidatif stres parametreleri (MDA, NO, SOD, CAT, GSH-Px, GR, GST ve GSH) analiz edildi. Çalışma sonunda kontrol grubu ile karşılaştırıldığında, DİO uygu- lanan grubun parametrelerinde herhangi bir değişiklik tespit edilmedi. EMB uygulanan grupta kontrole kıyasla tüm dokularda MDA ile NO seviyelerinde anlamlı bir artış kaydedildi. GSH düzeyleri ile GSH, GR, GST, SOD ve CAT enzim aktivitelerinde ise azalma olduğu belirlendi. Ayrıca serum AST, ALT, ALP, LDH, kolesterol, trigliserit, BUN, ürik asit ve kreatinin düzey/aktivitelerinde artış görülürken serum total protein ve albümin düzeylerinde azalma görüldü. EB ile DİO’nun birlikte uygulandığı grupların değerlerinin doza bağlı olarak kısmen ya da tamamen kontrol grubunun değerlerine yaklaştığı tespit edildi. Sonuç olarak, DİO’nun sıçanlarda EMB kaynaklı toksisiteye karşı koruma sağlayabileceği düşünülmektedir.

Anahtar Kelimeler:

Emamektin benzoat, diosmin, oksidatif hasar, rat

Investigation of the Protective Effects of Diosmin Against Emamectin Benzoate Induced Oxidative Damage in Rats

This study was aimed to investigate the effects of diosmin (DIO) in rats exposed to emamectin benzoate (EB). For this purpose, a total of 60 Wistar Albino male rats, aged 6 to 8 weeks and weighing 180 to 250 g, were used, 10 in each group. The groups were determined, respectively, control, EB (10 mg kg-1), DIO (50 mg kg-1), DIO (100 mg kg-1), EB (10 mg kg-1) + DIO (50 mg kg-1), EB (10 mg kg-1) + DIO (100 mg kg-1), and the indicated doses were applied by gavage for 21 days. At the end of the trial period, a heart puncture was performed under anesthesia and blood samples were taken into tubes with heparinized and anticoagulant-free properties. The liver, kidney, brain, testis, heart and lung tissues were removed after cervical dislocation. While some biochemical markers, such as aspartate aminotrans- ferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), alkaline phosphatase (ALP), triglyceride, cholesterol, total protein, albumin, uric acid, blood urea nitrogen (BUN) and, creatinine were assessed in the serum, oxidative stress indicators, such as MDA, NO, SOD, CAT, GSH-Px, GR, GST, and GSH, were determined in tissue samples. At the end of the investigation, no changes between the parameters of the DIO groups and the control group were discovered. Comparing the EB group to the control group, it was found that the levels of MDA and NO in tissues/ erythrocytes had significantly increased. The activity of the GSH, GR, GST, SOD, and CAT enzymes, as well as GSH levels, was found to have decreased. Furthermore, serum total protein and albumin levels were reduced, although AST, ALT, ALP, LDH, cholesterol, triglyceride, BUN, uric acid, and creatinine levels/activities were elevated. Depend- ing on the dose, it was found that the values of the groups coadministered with EMB and DIO were close to those of the control group. In conclusion, it is proposed that DIO could provide protection against EMB-induced toxicity in rats.

Keywords:

Emamectin benzoate, diosmin, oxidative damage, rat,

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