Ratlarda Pentaklorofenol Zehirlenmesinde Nar Çekirdeği Yağının Lipid Peroksidasyonu ve Biyokimyasal Parametrelere Etkileri

Çalışma Sprague-Dawley ırkı erkek sıçanlarda yapıldı. Çalışmada ilki kontrol olmak üzere 4 grup oluşturuldu. İkinci gruba 0.15ml/kg dozunda nar çekirdeği yağı (NÇY), üçüncü gruba 40mg/kg dozunda pentaklorofenol (PCP) ve dördüncü gruba 40mg/kg dozunda PCP+0.15ml/kg dozunda NÇY 28 gün boyunca gavaj ile uygulandı. Deneme sonunda eritrositte hemoglobin, katalaz ve glutasyon peroksidaz, nitrik oksit, malondialdehit, süperoksit dismutaz parametreleri; serumda 8-hidroksi-2-deoksi guanosin (8-OH-dG) ve biyokimyasal parametreleri incelendi. Sonuç olarak, bu çalışmada sıçanlara oral yoldan gavaj ile 40mg/kg uygulanan PCP’nin karaciğer hasarına, lipid peroksidasyonuna ve antioksidan enzim etkinliklerinde azalmaya yol açtığı; NÇY’nin PCP’nin istenmeyen etkileri üzerine koruyucu rolü olduğu belirlendi.

Anahtar Kelimeler:

Lipid peroksidasyon, nar çekirdeği yağı, pentaklorofenol, rat

Effects of Pomegranate Seed Oil on Pentachlorophenol Toxicity Lipid Peroxidation and Some Biochemical Parameters in Rats

In the study, Sprague-Dawley male rats were used and four groups were formed. The first group was heldas the control group. The second group was given 0.15 ml/kg pomegranate seed oil (PSO), the third group was given40mg/kg pentachlorphenol (PCP) and, the forth group was given 40mg/kg PCP+0.15 ml/kg PSO during 28 daysas specified into stomach by gavages. At the end of the experiment, erythrocyte haemoglobin, catalase, glutathioneperoxidase, nitric oxide, blood serum 8-hidroksi-2-deoksi guanosin (8-OH-dG) and biochemical parameters wereanalysed. As a result, 40mg/kg PCP applied by gavage, caused liver damage, lipid peroxidation and decreased theanti-oxidant enzyme activity and 0.15ml/kg PSD applied for 28 days, had no negative effect on protein, oil and carbonhidratemetabolism, had no toxic effect on liver and had no effect on lipid peroxidation. Overall, it’s determinedthat pomegranate seed oil prevents the negative effects of the pentachlorophenol.

Keywords:

Lipid peroxidation, pentachlorophenol, pomegranate seed oil, rat,

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