Tekin KECECI, Kadir Semih GUMUSSOY, Harun HIZLISOY

İnek Sütlerinden İzole Edilen Enterococcus faecalis ve Enterococcus faecium’un Vankomisin Direnci

Bu çalışmada çiğ inek sütlerinden Enterococcus spp’lerin izolasyonu ve identifikasyonu ile elde edilen izolatlarda vankomisin direncinin fenotipik ve moleküler yöntemlerle saptanması amaçlandı. Toplam 150 adet süt örneği sağlıklı ve mastitisli hayvanlardan California Mastitis Test ile skorlanarak toplandı. Seksen dört Enterecoccus spp. izolatı elde edildi ve bunların 57 (%68), 8 (%9) ve 19 (%23)’ü sırasıyla Enterococcus faecalis, Enterococcus faecium and Enterococcus spp. olarak polimeraz zincir reaksiyonu ile identifiye edildi. Etest ile izolatların hiçbiri vankomisine direnç göstermedi. Bununla birlikte, E. faecalis izolatlarının 11 (%19)’inde ve E. faecium izolatlarının 7(%88)’sinde VanB geni pozitif olarak tespit edildi. Ayrıca, 1 izolatta VanC2, VanC3 genleri ve 2 E. faecium izolatında VanB, VanC2, VanC3 genleri birlikte bulundu. Bu çalışmada inek sütlerinde önemli derecede Enterococcus spp. varlığı saptanmıştır. Vankomisin direncinin moleküler testlerle tespitinden dolayı bu test, antibiyotik direncin tespitinde daha etkili olduğu sonucuna varıldı

Anahtar Kelimeler:

Enterococcus spp., mastitis, PZR, sığır, süt, vankomisin

Vancomycin Resistance of Enterococcus faecalis and Enterococcus faecium Isolated from cattle milk

In this study the presence of Enterococcus spp. in raw cattle milk and the detection of the resistance to vancomycin of the isolates by using phenotypic and molecular methods were investigated. Totally, 150 milk samples were collected from healthy animals or animals with mastitis scored with California Mastitis Test. Eighty four Enterococcus spp. were isolated and 57 (68%), 8 (9%) and 19 (23%) of the isolates were identified by Polymerase Chain Reaction as Enterococcus faecalis, Enterococcus faecium and Enterococcus spp., respectively. None of the isolates were resistant to vancomycin with E test. However, 11 (19%) E. faecalis and 7 (88%) E. faecium isolates were positive for VanB. VanC2, VanC3 and VanB, VanC2, VanC3 genes were found together in 1 and 2 E. faecium isolates, respectively. In this study, Enterococcus spp. were significantly found in cattle milk. Because of the detection of vancomycin resistance by molecular test, this method was found to be more effective in the detection of antibiotic resistance.

Keywords:

Cattle, Enterococcus spp., mastitis, milk, PCR, vancomycin,

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