Ayhan ATASEVER, Yücel ÇAM, Fatma UYANIK, Kadir Semih GÜMÜŞSOY

Köpeklerde deneysel akciğer aspergillozis' i ve itrakonazol ile sağaltımı

Bu çalışmada, köpeklerde Aspergillus fumigatus ile intratrakeal yolla oluşturulan deneysel enfeksiyonda; klinik, hematolojik, biyokimyasal, idrar, histopatolojik ve mikolojik bulguların belirlenmesi ve itrakonazol'ün sağaltım etkinliğinin değerlendirilmesi amaçlandı. Çalışmada, 16 sağlıklı erkek köpek, enfekte kontrol ve sağaltım grubu olmak üzere iki eşit gruba ayrıldı. Aspergillus fumigatus ihokulasyonundan önce köpeklere siklofosfamid verildi. Daha sonra intratrakeal yolla A. fumigatus inoküle edildi. Etkenin verilişinin 5. gününde her iki grupta lökositosiz belirlendi. Serum ALP aktivitesi yükselirken, total protein, albumin ve globulin konsantrasyonları düştü. Köpeklerin akciğerler ve burun eküvyonundan A. fumigatus izole edilirken histopatolojik olarak sadece akciğerlerde granulomatöz yangı ve etken hifaları tespit edildi. Etken verilmesini takiben 7. günde sağaltıma başlandı. Sağaltım grubundaki köpeklere 10 mg/kg vücut ağırlığı/gün dozda 30 gün süreyle oral Itrakonazol verildi. Deneme sonunda, sağaltım grubunda bir köpekte gözlenen deri lezyonları ve enfekte kontrol grubunda iki köpekte gözlenen seröz burun akıntısı dışında tüm klinik belirtilerin düzeldiği belirlendi. Deneme öncesindeki değerlere göre, enfekte kontrol grubunda serum albumin konsantrasyonunda ve ALT aktivitesinde düşme, sağaltım grubunda serum ALT aktivitesinde yükselme, MCHC değerinde düşme ve total protein,.albumin ve globulin konsantrasyonlarında düşmenin devam etmesi dışında her iki grupta hematolojik ve biyokimyasal parametrelerin normale yakın olduğu tespit edildi. Enfekte gruptaki 2 köpeğin burun eküvyonu ve akciğerlerinden etken izole edilirken her iki gruptaki köpeklerde akciğerlerin histopatolojik olarak normal olduğu belirlendi.

Experimental pulmoner aspergillosis in dogs and treatment with itraconazole

The aim of this study was to determine the clinical, haematological, biochemical, urine, histopathological and my-cological findings and'to evaluate the efficiency of itraconazole treatment in experimentally infected dogs via intratracheal route with Aspergillus fumigatus. In study, 16 healthy male dogs were evenly divided into two groups as infected control and treated. Cyclophosphamide was injected to dogs before the inoculation of A. fumigatus. Then, A. fumigatus was inoculated via intratracheal route. On day 5 after the inoculation of the agent, leukocytosis were determined in both groups. Serum total protein, albumin and globulin concentrations were decreased while serum ALP activity was increased. Aspergillus fumigatus was isolated from nasal swaps and lungs of the dogs while the hyphae of the agent and granulomatous inflamation were observed only in lungs by histopathological examination. The treatment of animals was started on day 7 after the inoculation of the agent. Itraconazole, at 10 mg/kg body weight/day was administrated orally for 30 days. At the end of the experiment, all of the clinical signs in both groups were recovered except cutaneous lesions in one dog in the itraconazole treated group and serous nasal discharge in 2 dogs in the infected group. Compared to the beginning values of the experiment, haematological and biochemical parameters in both groups returned closely to normal values except the reductions in serum albumin concentrations and ALT activities in infected control group; elevation in serum ALT activity; decrease in MCHC; progressive reduction in total protein, albumin and globulin concentrations in itraconazole treated group. Significant histopathologic lesions in the lungs of the dogs were not seen in both groups while Aspergillus fumigatus was isolated from nasal swaps and lungs from only 2 dogs in the infected group.

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1.Arda M. 1980. Mikoloji. Ankara: A Ü Basımevi, p. 185-191.
2.Çam Y, Ataşe ver A, Uyanık F, Gümüşsoy KS, 2002. Köpeklerde deneysel Aspergillus fumigatus enfeksiyonu ve ıtrakonazol ile sağaltımı. I. Veteriner Patoloji Kongresi. Eylül, 12-13, Konya.
3.Dallman MJ, Dew TL, Tobias L, Doss R, 1992. Disseminated aspergillosis in a dog with diskospondylitis and neurologic deficits. J A V M A., 200:511-513.
4.Düzgüneş O, Kesici T, Gürbüz F. 1983. İstatistik Metotları A Ü Vet Fak Yay., 861. Ankara: A Ü Basımevi.
5.Espınebıngroff A, Bartlett M, Bowden R, Chin NX, Cooper C, 1997. Multicenter evaluation of proposed standardized procedure for antifungal susceptibility testing of filamentous fungi. J Clin Microbiol., 35: 139-143.
6.Gilbert JJ, 1998. Fungal infections. Susan EA, Asa M. Eds. The Merck Veterinary Manual. Eighth Edition, Philadelphia, Pennsylvania: A Merck and Rhöne-Poulenc Company, p. 459-474.
7.Helt MC, Riviere JME, 1995. Antifungal and antiviral drugs. Adams R. Eds. Veterinary Pharmacology and Therapeutics. 7th. ed., Ames: Iowa State University Press, p 855-883.
8.Kelly SE, Shaw SE, Clark WT, 1995. Long term survival of four dogs with disseminated Aspergillus terreus infection treated with itraconazole. Aust Vet J., 72:311-313.
9.Kurup VP, 1984. Interaction of Aspergillus fumiga tus spores and pulmonary alveolar macrophages of rabbits. Immunobiol, 166: 53-61.
10.Legendre AM, Rohrbach BW, Toal RL, Rinaldi MG, Grace LL, Jones JB, 1996. Treatment of blastomycosis with itraconazole in 112 dogs. J Vet Intern Med., 1996, 10:365.
11.Mandal PC, Gupta PP, 1993. Experiments asper gillosis in goats: clinical, haematological and my- cological studies. J Vet Med B., 40: 283-286.
12.Nichols PR, Morris DO, Beale KM, 2001. A retrospective study of canine and feline cutaneous vasculitis. Vet Dermatol, 12: 255-264.
13.Patterson TF, Fothergill AW, Rinaldi MG, 1993. Efficacy of itraconazole solution in a rabbit model of invasive aspergillosis. Antimicrob Agents Chemother., 37: 2307-2310.
14.Perez J, Mozos E, Lara FM, Paniagua J, Day MJ, 1996. Disseminated aspergillosis in a dog: an in munohistochemical study. J Comp Pathol, 115:191-196.
15.Schmitt HJ, Edwards F, Andrade J, Niki Y, Arm strong D, 1992. Comparison of azoles against as- pergilli in vitro and in an experimental model of pulmonary aspergillosis. Chemotherapy., 38: 118-126.
16.Southard C, 1987. Bronchopulmonary aspergillo sis in a dog. J A VM A., 190:875-877.
17.Taboada J, 2000. Systemic mycoses. Ettinger SJ, Feldman EC eds. Textbook of Veterinary Internal Medicine: Diseases of the Dog and Cat. 5 th ed, Vol. 1, Philadelphia, London, Toronto: W. B. Saunders Company, p. 453-476.
18.Thurston JR, Cysewski SJ, Richard JL, 1979. Ex posure of rabbits to spores of Aspergillus fumiga- tus or Penicillium sp: survival of fungi and micro scopic changes in the respiratory and gastrointesti nal tracts. Am J Vet Res., 40: 1443-1449.
19.Watt PR, Robins, GM, Galloway AM, O'boyle DA, 1995. Disseminated opurtifhistic fungal dis ease in dogs: 10 cases (1982-1990). J AV M A., 207:67-70.
20.Willis AM, Martin CL, Stiles J, 1999. Sino-orbital aspergillosis in a dog. J A V M A., 214: 1644- 1647.