Davut BAYRAM, Bilal AKYÜZ, Kaan M. İŞCAN, Okan ERTUĞRUL

Kayseri bölgesinde yetiştirilen şarole ırkı sığırlarda ve Türkiye yerli sığır ırklarında miyofosforilaz eksikliği (glikojen depo hastalığı tip v) allelinin PCR-RFLP ile belirlenmesi

Bu çalısmanın amacı Türkiye’de yetistirilen saf ve melez Sarole ırkı sıgırlar ile Türkiye yerli sıgır ırklarında miyofosforilaz eksikligi allelinin bulunup bulunmadıgının arastırılmasıdır. Arastırmada, on bas Sarole, 15 bas Sarole- Simmnetal melezi, 20 bas Yerli Kara, 20 bas Boz Irk, 20 bas Güney Anadolu Kırmızısı, 20 bas Dogu Anadolu Kırmızısı ve kontrol grubu olarak da 20 bas Simmental sıgırı toplam 125 sıgır kullanılmıstır. PCR ürünleri StyI restriksiyon enzimi ile kesilmistir. Elde edilen parçacıklarda StyI restriksiyon bölgesinin varlıgı veya yoklugu % 3’lük NuSieve agaroz jel elektroforezi ile bakılmıstır. Incelenen Sarole, Sarole melezi ve yerli sıgırlarda miyofosforilaz eksikligi alleline rastlanılmamıstır.

Detection of myophosphorylase deficiency (glycogen storage disease type v) with PCR-RFLP in charolais cattle in Kayseri region and in Turkey Native cattle breeds

The aim of this study was to investigate whether or not the myophosphorylase deficiency allele exists in Charolais and in Turkish native cattle breeds in Turkey. Ten Charolais, 15 Charolais-Simmental crossbreds, 20 Native Black, 20 Gray Steppe, 20 South Anatolian Red, 20 East Anatolian Red and 20 Simmental breed for the control group comprised the 125 cattle used in this study. The PCR products were digested by StyI restriction enzyme. The resulting fragments were analyzed on 3 % NuSieve agarose gel for the presence or absence of a StyI restriction site. There were no positive results indicating the presence of myophosphorylase deficiency allele in Charolais, Charolais crossbreed and Turkish native cattle breeds.

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