Kantitatif RT-PCR (RT-qPCR) ile MikroRNA (miRNA) Ekspresyon Profillemesi

Gen ekspresyonunun post-transkripsiyonel düzenleyicisi olarak bilinen miRNA’lar ökaryotik canlılarda çeşitli fizyolojik ve patolojik süreçlerde rol oynamaktadır. miRNA'ların rollerinin ortaya konulması ile birlikte miRNA’lar üzerine yapılan çalışmaların sayısı da gün geçtikçe artmaktadır. Yapılan çalışmalar ile birlikte miRNA'ların bütün hücre ve doku tiplerinde eksprese olduğu ortaya konmuştur. miRNA ekspresyon profilinin ortaya konması için çeşitli yöntemler öneril-mesine rağmen, yüksek duyarlılığı ve özgüllüğü nedeniyle RT-qPCR altın standart olarak kabul edilmektedir. Bu derle-mede; RT-qPCR ile miRNA ekspresyon profillemesi sürecindeki adımlar detaylı olarak özetlenmiş ve konu ile ilgili lite-ratür bilgisine yer verilmiştir.

Anahtar Kelimeler:

Ekspresyon, miRNA, normalizasyon, RT-qPCR

MicroRNA (miRNA) Expression Profiling by Quantitative RT-PCR (RT-qPCR)

miRNAs, known as post-transcriptional regulators of gene expression, play a crucial role in various physio-logical and pathological processes in eukaryotic organisms. As the roles of miRNAs have been revealed, the number of studies on miRNAs is increasing day by day. Studies have shown that miRNAs are expressed in all cell and tissue types. Although various methods have been proposed to demonstrate miRNA expression profile, RT-qPCR is consid-ered as a gold standard technique due to its high sensitivity and specificity. In this review; the steps in the miRNA ex-pression profiling process by RT-qPCR were summarized in detail and relevant literature knowledge was given.

Keywords:

Expression, miRNA, normalization, RT-qPCR,

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