Kanatlı hayvanlarda Bordetella avium'a karşı oluşan antikorların mikroaglutinasyon (MAT) indirekt fluoresan antikor (IFA) ve ELISA (Enzyme Linked Immunosorbent Assay) teknikleri ile saptanması

Bu çalışmada, hindi ve broylerlerde B. avium infeksiyonunun varlığı ELISA, MAT ve IFAT teknikleriyle araştırıldı. Bu amaçla Bolu, İzmir, Bigadiç yörelerinden 1050 hindi ve Ankara çevresinden 500 broyler serum örneği incelendi. Çalışma sonucu hindi serum örneklerinin ELISA ile %27.1, MAT ile %21.0, IFAT ile %17.3'ü pozitif, broyler serum ör¬neklerinin ise ELISA ile %14,2, MAT ile %9.6, IFAT ile %7.2'si pozitif bulunmuştur. Sonuç olarak bu araştırmada ELISA ile B. avium'a karşı daha fazla sayıda seropozitiflik saptandı. Bununla birlikte IFAT ve MAT tekniklerinin de hastalığın indirekt tanısında kullanılabileceği gösterildi.

Detection of antibodies in poultry produced against Bordetella avium by microagglutination (MAT) indirect fluorescence antibody (IFA) and ELISA (Enzyme Linked Immunosorbent Assay) techniques

In this study B.av/iv/rrinfections were detected in broiler and turkey with ELISA, MAT and IFAT techniques. For this aim, 1050 turkey sera samples collected from Bolu, Izmir, Bigadiç regions and 500 broiler sera samples collected from Ankara region were analysed. It was found that 27.1%, 21.0% and 7.2% of turkey sera samples were seropositive with ELISA, MAT and IFAT techniques, respectively, whereas, 14.2%, 9.6% and 17.3% of broiler sera samples were seropositive with ELISA, MAT and IFAT techniques, respectively. Consequently, more seropositive results are obtained with ELISA technique, However, IFAT and MAT techniques can be used for the indirect diagnose of 0. avium infections.

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