2015

Ankara tavşanı derisinde S100 proteini ve altüniteler S100$alpha$ ile S100$beta$'nın immunohistokimyasal lokalizasyonu

S100 proteinleri, farklı hedef proteinlerle etkilesime girerek bir çok hücresel olayda görev alan kalsiyum baglayıcı proteinlerdir. Ankara tavsanı derisinde S100 proteini ve bu proteinin altüniteleri olan S100$alpha$ ve S100$beta$’nın varlıgı ve hücresel lokalizasyonları ile ilgili herhangi bir arastırma bulunmamaktadır. Çalısmada materyal olarak on adet saglıklı, eriskin, beyaz Ankara tavsanından alınan sırt derileri kullanıldı. S100 proteinlerinin lokalizasyonunun belirlenmesi amacıyla strept-ABC immunohistokimyasal boyama yöntemi uygulandı. Immunohistokimyasal boyamalarla, Langerhans hücrelerinin, melanositlerin, dermal dendritik hücrelerin, kıl follikülü iç katmanını olusturan hücrelerin, kıl bulbusundaki bazı hücrelerin ve kan damarlarının S100$beta$ alt ünitesini, dermiste bulunan muskulus arrektor pili ve çizgili kasların ise S100$alpha$ alt ünitesini içerdikleri gözlendi. Bununla birlikte, sinir teli demetlerinin her iki molekülü de içerdigi, keratinositlerin ise içermedikleri dikkati çekti. S100 proteini ve bu proteinin alt üniteleri olan S100$alpha$ ile S100$beta$’nın Ankara tavsanı derisindeki varlıgı ve proteinlerin hücresel lokalizasyonları ilk kez bu çalısmayla ortaya konulmustur.

Immunohistochemical localization of S100 protein and subunits S100$alpha$ and S100$beta$ in the skin of Angora rabbit

S100 proteins are calcium binding proteins, which play significant roles in various cellular activities, via interacting with different target cellular proteins. There is no available information about the localization of S100 proteins in the skin of Angora rabbit. In this study, the skin patterns taken from back region of ten healthy adult white Angora rabbits were used as a material. To determine of the localization of S100 proteins, a strept-ABC immunohistochemical staining procedure was applied. The Langerhans cells, melanocytes, dermal dentritic cells, inner cells of hair follicles, some cells in hair bulby, blood vessels and neural structures contained the subunit S100$beta$ whereas the arrector pili muscle and striated muscle cells in the dermis contained the subunit S100$alpha$. Both molecules were expressed by neural fibers but not the keratinocytes. The authors claim that the presence and cellular localization of S100 protein and its subunits S100$alpha$ and S100$beta$ in the skin of Angora rabbit have been firstly elucidated in this study.

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