Phytophthora capsici Leon. ile enfekte edilen duyarlı ve dayanıklı biber genotiplerinin köklerinde antioksidatif tepkiler ve lipid peroksidasyonu

Phytophthora capsici’ye dayanıklılıkları faklı olan üç biber genotipi [dayanıklı PM-702, duyarlı Kahramanmaraş-Acı (KM-Acı) ve Demre-8] peroksidaz (EC 1.11.1.7) aktivitesini, fenolik bileşikler ve bir lipid peroksidasyon ürünü olan MDA (malondialdehit)’yı analiz etmek için farklı zoospor konsantrasyonları (102, 103 ve 104 zoospor/ml) ile inokule edilmiştir. Enfeksiyondan sonra altıncı günde biber genotiplerinin köklerinde zoospor konsantrasyonlarına bağlı olarak peroksidaz aktivitesinde önemli farklılıklar gözlenmiştir. İnokule edilen dayanıklı PM-702 genotipinde peroksidaz aktivitesi artmış ve kontrol gruplarındaki en yüksek peroksidaz aktivitesi dayanıklı PM-702 genotipinde saptanmıştır (P

Anahtar Kelimeler:

Antioksidan, biber, fenolik, MDA, peroksidaz, P.capsici

Antioxidative Reactions and Lipid Peroxidation in Roots of Susceptible and Resistant Pepper Genotypes infected with Phytophthora capsici Leon

Three pepper genotypes (resistant PM-702, susceptible Kahramanmaraş- Hot (KM-Hot) and Demre-8) with, different resistance to Phytophthora capsici were inoculated with different concentration of zoospores (102, 103 and 104 zoospore/ml) to analyze of peroxidase (EC 1.11.1.7) activity, phenolic compounds and MDA (Malondialdehyde) which is a product of lipid peroxidation. Samples were collected from the roots of three pepper genotypes on the 6th days after infection. Important differences were observed in peroxidase activity with respect to zoospore concentration and type of pepper genotypes. Activity of peroxidase was increased in inoculated roots of resistant PM-702 genotype and the highest peroxidase activity in non-infected (control) roots was recorded in resistant genotype PM-702. Although, phenolic compounds were increased in PM-702 and KM-Hot in relation to inoculum concentrations. It was decreased in Demre-8 genotype. When all three pepper genotypes were compared in terms of MDA content on roots. Amount of MDA in the roots of DEM-8 genotype was significantly higher than those of PM-702 and KM-Hot genotypes (P<0.01).

Keywords:

Antioxidant, pepper, phenolic, MDA, peroxidase, P.capsici,

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