Burcu AKTAŞ, Hatice BASMACIOĞLU MALAYOĞLU

Üzüm çekirdeği, Biberiye, Yeşil çay ve Zeytin yaprağı Ekstraktlarının Fenolik Bileşikleri ve Antioksidan Aktiviteleri

Bu çalışmada, üzüm çekirdeği, biberiye, yeşil çay ve zeytin yaprağı etanol ekstraktlarının toplam antioksidan aktiviteleri ve fenolik bileşikleri araştırılmıştır. Bitkisel ekstraktların toplam antioksidan aktiviteleri 2.2-difenil-1-pikrilhidrazi hidrat (DPPH) radikal süpürme aktivitesi ve troloks eşdeğeri antioksidan kapasitesi (TEAC) olmak üzere iki farklı yöntemle analiz edilmiştir. Biberiye ekstraktı (BE) karnosik asit ve rosmarinik asit, yeşil çay ekstraktı (YE) toplam kateşinler ve zeytin yaprağı ekstraktı (ZYE) oleuropein içerikleri, HPLC yöntemi ile tespit edilmiştir. Üzüm çekirdeği ekstraktı (ÜÇE) kondanse tanenleri ise Butanol-HCl yöntemi ile analiz edilmiştir. Bitkisel ekstraktların Folin-Ciocaltaeu yöntemine göre saptanan toplam fenol içerikleri 141.7 ile 315.56 mg mg of gallik asit eşdeğeri (GAE) g-1 arasında değişim göstermiştir. Bu çalışmadan elde edilen bulgulara göre; biberiye ekstraktı en yüksek radikal süpürme aktivitesi gösterirken, yeşil çay ekstraktı en yüksek TEAC aktivitesi göstermiştir. Bununla birlikte, elde edilen verilerin toplam fenol içeriği ile TEAC arasında yüksek doğrusal korelasyon (R=0.82) ve toplam fenol içeriği ile DPPH arasında düşük doğrusal korelasyon (R=0.47) saptanmıştır.

Anahtar Kelimeler:

Bitkisel ekstraktlar, antioksidan aktivite, fenolik bileşikler, DPPH

Comparison of Phenolic Compounds and Antioxidant Activities of the Extracts of Grape Seed, Rosemary, Green Tea and Olive Leaf

Objective: Total antioxidant activities and phenolic compounds of ethanol extract of grape seed, rosemary, green tea and olive leaf were investigated in this study.Material and Methods: The total antioxidant activities of plant extracts were analyzed by two different methods, including 2.2-diphenyl-1-picrylhydrazyl hydrate (DPPH) radical scavenging activity and trolox equivalent antioxidant capacity (TEAC). Carnosic acid, rosmarinic acid in rosemary extract (RE), total catechins in green tea extract (GTE) and oleuropein in olive leaf extract (OLE) were identified by high performance liquid chromatography (HPLC) method. Condensed tannins of grape seed extract (GSE) was analyzed by butanol-HCl method.Results: The total phenolic contents of plant extracts determined by the Folin-Ciocalteu method ranged from 141.7 to 315.56 mg of gallic acid equivalents (GAE) g-1. According to results obtained this study, RE exhibited the highest DPPH radical scavenging activity, and GTE exhibited highest TEAC activity. Additionally, obtained data revealed high linear correlation (r=0.82) between total phenolic contents and TEAC values and low linear correlation (r=0.47) between total phenolic contents and DPPH values.Conclusion: The potent antioxidant activity of plant extracts provided from agro-industrial by-products, medicinal and aromatic plants makes it possible used them as natural sources of antioxidants in feed, food and pharmaceutical industries.

Keywords:

Plant extracts antioxidant activity, phenolic compounds, DPPH, TEAC,

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