Mehmet BEŞKEÇİLİ, Nihan GÜNEŞ, Mustafa GÜMÜŞ

Antalya ili Demre ilçesi biber yetiştiriciliğinde Tomato spotted wilt virus (TSWV) ve Cucumber mosaic virus (CMV) etmenlerinin yaygınlığının belirlenmesi

Amaç: Bu çalışmada Antalya ili biber üreticiliğinin yoğun olarak gerçekleştirildiği Kumluca ilçesinde serada yetiştirilen biber bitkilerinde TSWV ve CMV varlığının serolojik olarak belirlenmesi ve DAS-ELISA testi sonucunda pozitif olduğuna karar verilmesinde uygulanan kriterlerin doğruluğunun RT-PCR yöntemi ile karşılaştırılması amaçlanmıştır. Materyal ve Yöntem: Antalya ili Demre ilçesinde örtü altı biber üretim alanlarında sörveyler yapılarak virüs benzeri belirti gösteren bitkilerden örnekler toplanmıştır. Örnekler CMV ve TSWV etmenlerine spesifik monoklonal ve/veya poliklonal antibody’ler kullanılarak DAS-ELISA testine tabi tutulmuştur. ELISA okuyucuda farklı absorbans değerlerine sahip bazı pozitif örneklere etmenlere spesifik primerler kullanılarak RT-PCR testleri uygulanmıştır. Araştırma Bulguları: DAS-ELISA yöntemi sonucuna göre toplanan 82 örnekten 65 tanesinde (%79) en az bir virüs etmenine rastlanılmıştır. Sadece CMV olan örnek sayısı 10 adet (%12) olup sadece TSWV olan örnek sayısı 47 adet (%57) olarak tespit edilmiştir. 8 örnekte (%10) hem TSWV hem de CMV’nin karışık infeksiyon yaptığı belirlenmiştir. DAS-ELISA sonucunda pozitif olduğu tespit edilen örneklerin ELISA okuyucuda farklı absorbans değerlerine sahip pozitif sonuç veren örneklerde etmenlerin varlığı RT-PCR yöntemi ile doğrulanmıştır. Sonuç: Biber örneklerinde TSWV etmeninin daha fazla bulunduğu CMV etmeninin ise fazla yaygınlık göstermediği belirlenmiştir.

Anahtar Kelimeler:

Cucumber mosaic virus (CMV), DAS-ELISA, RT-PCR, Tomato spotted wilt virus (TSWV)

Determination and prevalence of Tomato spotted wilt virus (TSWV) and Cucumber mosaic virus (CMV) in pepper production areas in Demre district of Antalya

Objective: In this study, it was aimed to serological determination of the presence of TSWV and CMV on pepper plants grown in the greenhouses in Kumluca district where Antalya pepper production is carried out intensely and compare the accuracy of the criteria determining that positive applied in by DAS-ELISA with RT-PCR method. Material and Methods: Samples were collected from virus-like symptoms showing plants by conducting surveys in greenhouse pepper production areas in the Demre district of Antalya province. Samples were subjected to DAS-ELISA test using monoclonal and /or polyclonal antibodies specific for CMV and TSWV. RT-PCR tests were applied to some positive samples which have different absorbance values in the ELISA reader by using specific primers. Results: According to the results of the DAS-ELISA method, 65 of the 82 samples (79%) were found to have at least one virus. The number of samples infected only with CMV was 10 (12%) and the number of samples infected only with TSWV was 47 (57%). In 8 samples (10%) were found to have both TSWV and CMV mixed infections. The presence of the factors in the samples which have positive absorbance values in the ELISA reader were compared by RT-PCR method and the presence of the factors was confirmed. Conclusion: It was determined that TSWV factor was found more in pepper samples and CMV factor did not show much prevalence.

Keywords:

Cucumber mosaic virus (CMV), DAS-ELISA, RT-PCR, Tomato spotted wilt virus (TSWV),

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Adkins, S. 2000. Tomato spotted wilt virus-positive steps towards negative success, Molecular Plant Pathology, 1(3):151-157 pp.
Arli-Sokmen, M.A., Mennan, H., Sevik, M.A. and Ecevit, O. 2005. Occurrence of viruses in field-grown pepper crops and some of their reservoir weed hosts in Samsun, Turkey, Phytoparasitica, 33(4):347-358pp.
Bozokalfa, M ve Eşiyok, D. 2010.Biber (Capsicum annuum L.) aksesyonlarında genetik çeşitliliğin agronomik özellikler ile belirlenmesi, Ege Üniversitesi Ziraat Fakültesi Dergisi, 47(2):123-134 s.
Burgmans, J.L., Fry, P.R. and Sunde, R. G. 1986. Peppers: survey of virus diseases of Capsicum annuum in Hawke’s Bay and Poverty Bay, New Zealand Journal of Experimental Agriculture, 14:459-463 pp.
Clark, M.F. and Adams, A.N. 1977. Characteristic of microplate method of Enzyme-Linked Immunosorbent Assay for detection of plant viruses. Journal of General Virology, 34:475-483 pp.
Deligoz, I., Sokmen, M.A. and Sari, S. 2014. First report of resistance breaking strain of Tomato spotted wilt virus (Tospovirus; Bunyaviridae) on resistant sweet pepper cultivars in Turkey, New Disease Reports, 30:26 p.
Espín, A., Alcázar, M.D., Egea, C. and Candela, M.E. 1994. Defensive response of two Capsicum annuum cultivars of different sensitivity to an isolate of Cucumber mosaic virus (CMV), Fitopatología, 29(3):178-187 pp.
Faggioli, F., Ferretti, L., Albanese, G., Sciarroni, R., Pasquini, G., Lumia, V. and Barba, M. 2005. Distribution of olive tree viruses in Italy as revealed by one-step RT-PCR. Journal of Plant Pathology, 49-55 pp.
Fanigliulo, A., Comes, S., Pacella, R., Crescenzi, A., Momol, M. T., Olson, S. M. and Reitz, S. 2007. Integrated management of viral diseases in field-grown tomatoes in southern Italy. In II International Symposium on Tomato Diseases 808:387-392 pp.
FAO, 2019. Food and Agriculture Organization of the United Nations, http://faostat3.fao.org/download/Q/QC/E (Erişim Tarihi: 26.Ocak 2019)
Foissac, X., Svanella-Dumas, L., Dulucq, M.J., Candresse, T. and Gentit, P. 2001. Polyvalent detection of fruit tree tricho, capillo and foveaviruses by nested RT-PCR using degenerated and inosine containing primers (PDO RT-PCR), Acta Hortic, 550:37-43 pp.
Gera, A., Kritzman, A., Cohen, J., Raccah, B. and Antignus, Y. 2008. Tospoviruses infecting vegetable crops in Israel, Eppo Bulletin, 30(2):289-292 pp.
Gitaitis, R.D., Dowler, C.C. and Chalfant, R.B. 1998. Epidemiology of Tomato spotted wilt virus in pepper and tomato in Southern Georgia, Plant Disease, 82:752-756 pp.
Gracia, O., de Borbon, C. M., Granvel de Millan, N. and Cuesta, G. V. 1999. Occurence of different Tospovirus in vegetable crops in Argentina, Journal of Phytopatghology, 147:223-227 pp.
Güneş, N. and Gümüş, M. 2019 Detection and Characterization of Tomato spotted wilt virus and Cucumber mosaic virus on Pepper Growing Areas in Antalya. Journal of Agricultural Sciences, 25(3), 259-271.
Hanssen, I.M., Lapidot, M. and Thomma, B.P.H.J. 2010. Emerging viral diseases of tomato crops, Molecular Plant-Microbe Interactions, 23:539-548 pp.
Kucharek, T.A., Purcifull, D.E., Christie, R.G. and Perkins, K.D. 1998. The association of severe epidemics of cucumber mosaic in commercial fields of pepper and tobacco in north Florida with inoculum in Commelina benghalensis and C. communis, Plant Disease, 82(10):1172 p.
Latham, L.J. and Jones, R.A.C. 1997. Occurrence of Tomato spotted wilt tospovirus in native flora, weeds and horticultural crops, Australian Journal of Agricultural Research, 48(3):359-369 pp.
Lobenstein, G. and Lecoq, H. 2012. Viruses and virus diseases of the vegetables in the Mediterraneam Basin, Academic Press-Elsevier, Amsterdam, 595p.
Margaria, P., Ciuffo, M. and Turina, M. 2004. Resistance breaking strain of TSWV Tospovirus, (Bunyaviridae) on resistant pepper cultivars in Almeria Spain, Plant Pathology, 53:795 p.
Mnari Hattab, M., Ezzaier, K., Gebre Selassie, K., Marchoux, G. and Gognalon, P. 1999. Surveys of viruses affecting pepper (Capsicum annuum L.) in Tunisia, Abstracts of papers presented at the VIIth International Plant Virus Epidemiology Symposium, Almeria, 143-144 pp.
Nour, S.M., Maleki, M. and Ghotbi, T., 2013, Biological and serological detection of TSWV on three commercial cultivars Chrysanthemum morifolium in Markazi province of Iran, Annals of Biological Research, 4(4):112-119 pp.
Perry. K.L., Zhang, L. and Palukaitis, P. 1998. Amino acid changes in the coat protein of Cucumber mosaic virus differentially affect transmission by the aphids Myzus persicae and aphis gossypii, Virology, 242:204-210 pp.
TÜİK, 2017. ‘‘Bitkisel Üretim İstatistikleri’’ https://biruni.tuik.gov.tr/bitkiselapp/ bitkisel.zul (Erişim tarihi: 26 Ocak 2019).
Uzunoğulları, N. ve Gümüş, M. 2015. Marmara bölgesi’nde bazı kültür bitkilerinde doğal enfeksiyona neden olan hıyar mozaik virüsü (Cucumber mosaic virus, CMV)’nün tespiti, Trakya University Journal of Natural Sciences, 16(1):9-15 pp.
Vozelj, N., Petrovic, N., Novak, M.P., Tusek, M., Mavric, I. and Ravnikar, M. 2003. The most frequent viruses on selected ornamental plants and vegetables in Slovenia, Zbornik predavanj in referatov 6.Slovenskega Posvetovanje o Varstvu Rastlin, Zrece, 300-304 pp.
Yardımcı, N. ve Çulal Kılıç, H. 2009. Tomato spotted wilt virus in vegetable growing areas in the West Mediterranean region of Turkey, African Journal of Biotechnology, 8(18):4539-4541 p.
Zitikaitė, I. and Samuitienė, M. 2009. Detection and characterization of Cucumber mosaic virus isolated from sweet peppers, Sodininkystė ir Daržininkystė, 28(3):281-288 pp.
Zitter, T.A. and Murphy, J.F. 2009. Cucumber mosaic virus, The Plant Health Instructor, DOI: 10.1094/PHI-I-2009-0518-01.