Yasemin SEZGİN, Mehtap BİLGİN ÇETİN, Özlem Nilgün ALPTEKİN, Şule BULUT, Özlem DARCANSOY İŞER, Yunus Kasım TERZİ

Farklı Resveratrol Konsantrasyonlarının Gingival Fibroblastlardaki Hücre Canlılığına Etkisi

Amaç: Bu çalışmanın amacı, farklı resveratrol konsantrasyonlarının gingival fibroblast canlılığı üzerindeki etkisini MTT (3-[4,5-dimetiltiyazol-2-yl]-2,5-difeniltetrazolyum bromür) yöntemiyle belirlemektir. Yöntem: Çalışmamızda, fibroblast kültürlerinin kurulmasında sıçanların mandibular 1.molar dişlerinin bukkalinden alınan dişeti dokuları kullanıldı. Dokular mekanik olarak parçalanarak kültür kaplarına aktarıldı. Hücreler, DMEM besi ortamı içerisinde kültüre edildi. Resveratrolün hücre proliferasyonu üzerindeki etkisinin belirlenmesi için 4. pasaj kültürler kullanıldı. Hücre proliferasyonu, 3-[4,5-dimetiltiyazol-2-yl]-2,5-difeniltetrazolyum bromür (MTT) yöntemiyle ½ dilüsyonile 0,78-400µM konsantrasyon aralığında ve 48, 72 ve96 saat inkübasyon sürelerinin ardından incelendi. Sonuçların istatistiksel değerlendirmesinde tek yönlü ANOVA testinin Tukey ikili karşılaştırma yöntemi kullanıldı. Hücrelerin %50 canlılık gösterdiği inhibisyon konsantrasyonları (IC50) logaritmik canlılık eğrilerinden hesaplandı. Bulgular: 0,78-25µM konsantrasyon ve 72 ile 96 saat inkübasyon süresi aralığında fibroblast canlılığının %85’in üzerinde; 48 saat sonra ise %90’ın üzerinde olduğu saptandı. Uygulanan çözücünün hücre canlılığı üzerinde herhangi bir etkisinin olmadığı belirlendi. IC50 değerleri 48, 72 ve 96 saatler için sırasıyla 120,1±5,7 µM; 85,2±6,4 µM; 105,9±7,1 µM olarak belirlendi. Sonuç: Bu doz belirleme çalışmasının sınırları dahilinde, 0,78-25µM resveratrol konsantrasyonunun fibroblast canlılığı üzerinde olumsuz etkisi olmadığı söylenebilir.

Effect of Different Resveratrol Concentrations on Cell Viability on Gingival Fibroblasts

Objectives: The aim of this study was to investigate the effect of different concentrations of resveratrol on gingival fibroblast viability with (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay. Methods: In our study rat buccal gingival tissues from the area surrounding the mandibular first molar were used for establishment of fibroblast cultures. Rat gingival tissues were mechanically disrupted, and cultured in DMEM supplemented with 15% fetal bovine serum, 2 mM L- glutamine, and penicillin (100 IU/mL)/streptomycin (100 μg/mL). The 3-[4,5-dimethylthiazol-2-yl]-2,5- diphenyltetrazolium bromide (MTT) assay and forth passage cultures were used for evaluating the effects of resveratrol on fibroblast viability in a concentration range of 0,78-400µM after 48, 72 and 96 hours of incubation. Inhibitory concentration 50 (IC50) values were calculated from the logarithmic trend lines of the proliferation versus concentration graphs. Results: MTT assay showed that viability of gingival fibroblast cells were over 85% and 90% after 72-96 hours and 48 hours respectively in a 0.78-25µM concentration range of resveratrol. IC50 were 120,1±5,7 µM; 85,2±6,4 µM; 105,9±7,1 µM for 48, 72, 96 hours, respectively. Conclusion: Within the limitations, it can be concluded that 0.78-25µM concentration range of resveratrol doesn’t have negative effect on fibroblast viability.

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