Beral AFACAN, Zeynep Pınar KELEŞ YÜCEL, Çiğdem YENİSEY, Nejat NİZAM, Gülnur EMİNGİL

Salivary Resistin and TNF-α Levels in Different Periodontal Diseases

Objective: Resistin is a cytokine involved in insulin resistance, inflammation and immunity. The relationship of resistin with tumor necrosis factor (TNF)-α, one of the key cytokines of periodontal inflammation, is bidirectional. This study aimed to evaluate salivary resistin and TNF-α levels in different periodontal diseases. Methods and Materials: Saliva samples were collected from 20 generalized aggressive periodontitis (G-AgP), 20 chronic periodontitis (CP), 20 gingivitis patients and 20 periodontally healthy individuals. Probing pocket depth (PD), clinical attachment levels (CAL), papillary bleeding index (PBI) and plaque index were recorded. Salivary resistin and TNF-α levels were analyzed by enzyme-linked immunosorbent assay. Inter-group comparison was performed by the Kruskal-Wallis test and Dunn’s post-hoc test. Results: G-AgP, CP and gingivitis groups exhibited higher salivary resistin and TNF-α levels compared to the periodontally healthy controls, however, this did not reach to statistical significance (p>0.05). Although both periodontitis groups had significantly higher mean PD, CAL and PBI scores compared to gingivitis group (p0.05). Conclusion: Within the limits of the present findings, salivary resistin and TNF-α levels could not differentiate periodontal disease from health. Further investigations with larger sample size are needed to elucidate the potential relationship salivary levels of these cytokines with periodontal inflammation.

Farklı Periodontal Hastalıklarda Tükürük Resistin ve TNF-α Seviyeleri

Amaç: Resistin, insülin direnci, enflamasyon ve immünite ile ilişkili bir sitokindir. Resistinin, periodontal enflamasyonun ana sitokinlerinden biri olan tümör nekroz faktör (TNF)-α ile olan ilişkisi çift yönlüdür. Bu çalışma farklı periodontal hastalıklarda tükürük resistin ve TNF-α seviyelerini değerlendirmeyi amaçlamaktadır. Yöntem: Tükürük örnekleri, 20 yaygın agresif periodontitis (Y-AgP), 20 kronik periodontitis (KP), 20 gingivitis hastası ile 20 periodontal sağlıklı bireyden elde edildi. Sondalanan cep derinliği (SCD), klinik ataşman seviyesi (KAS), papil kanama indeksi (PKİ) ve plak indeksi değerleri kaydedildi. Tükürük resistin ve TNF-α seviyeleri enzim bağlı immunosorbent yöntemi ile analiz edildi. Gruplar arası karşılaştırmalar, Kruskal-Wallis testi ve Dunn post-hoc testi ile yapıldı. Bulgular: Y-AgP, KP ve gingivitis gruplarının tükürük resistin ve TNF-α seviyeleri, periodontal sağlıklı bireylerden yüksekti ancak bu fark istatistiksel olarak anlamlı bulunmadı (p>0.05). Her iki periodontitis grubunun SCD, KAS ve PKİ ortalama değerleri, gingivitis grubundan anlamlı yüksek olmasına rağmen (p0.05). Sonuç: Bu çalışmanın sınırlamaları dahilinde, tükürük resistin ve TNF-α seviyeleri periodontal hastalığı, periodontal sağlıktan ayıramamıştır. Bu sitokinlerin tükürükteki seviyelerinin periodontal enflamasyon ile olan olası ilişkisini aydınlatmak için daha geniş örneklem büyüklüğüne sahip ileri çalışmalara ihtiyaç vardır.

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