Pınar GÜMÜŞ, VELİ ÖZGEN ÖZTÜRK, Georgios BELİBASAKİS, Nagihan BOSTANCI, GÜLNUR EMİNGİL

Evaluation Of Salivary IL-1Beta And IL-6 Levels In Pregnant And Postpartum Women

Amaç: Gebelikte ortaya çıkan fizyolojik değişiklikler lokal enflamatuvar medyatörleri etkileyebilir ve periodontal enflamasyonun şiddetini arttırabilir. Çalışmamızda aynı kadında gebelikte ve doğum sonrasında tükürük IL-1beta (interlökin-1beta) ve IL-6 (interlökin-6) düzeylerinin incelenmesi ve periodontal hastalık şiddeti ile ilişkisinin değerlendirilmesi amaçlanmıştır. Yöntem: Toplam 96 gingivitis tanısı konan kadın çalışmaya dahil edilmiştir. Gebe kadınlar doğum sonrası 6. ayda tekrar değerlendirilmiş, gebe grubu ve doğum sonrası olmak üzere iki grup oluşturulmuştur. Tükürük IL-1beta ve IL-6 düzeyleri ELISA yöntemi ile belirlenmiştir. Parametreler arasında ki korelasyonlar Spearman's korelasyon analizi ile değerlendirilmiştir. Bulgular: Tükürük IL-1beta düzeyleri doğum sonrası grubunda gebe gruba göre anlamlı olarak düşük bulunmuştur (p=0.045). Doğum sonrası grupta IL-6 seviyeleri gebe grubundan anlamlı olarak yüksek düzeydedir (p

Gebelik Ve Doğum Sonrası Tükürük IL-1Beta And IL-6 Seviyelerinin Değerlendirilmesi

Introduction: Local inflammatory mediators could be affected from physiological changes in the pregnancy and exacerbate the periodontal inflammation. The aim of the present study was to evaluate pregnant and postpartum saliva levels of IL-1beta (interleukin1beta) and IL-6 (interleukin 6) levels in the same women and to investigate their association with periodontal disease severity. Methods: A total number of 96 women that diagnosed as gingivitis were originally recruited for this longitudinal study, assigned to the following groups; pregnant and postpartum group: the pregnant group re-evaluated 6 months after giving birth. IL-1β[?]and IL-6 levels were detected by ELISA. Correlations between parameters were analyzed by Spearman's correlation test. Results: Salivary IL-1beta levels were significantly lower in post-partum group than pregnant group (p=0.045). The post-partum group exhibited significantly higher IL-6 levels than pregnant group (p

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1. Armitage GC. Development of a classification system for periodontal diseases and conditions. Ann Periodontol 1999; 4: 1-6.
2. Sun Y, Guo QM, Liu DL, Zhang MZ, Shu R. In vivo expression of Toll-like receptor2, Toll-like receptor 4, CSF2 and LY64 in Chinese chronic periodontitis patients. Oral Dis 2010; 16: 343- 350.
3. Otomo-Corgel J, Pucher JJ, Rethman MP, Reynolds MA. State Of The Science: Chronic Periodontitis And Systemic Health. J Evid Base Dent Pract 2012; 20-28.
4. Armitage GC. Bi-directional relationship between pregnancy and periodontal disease. Periodontology 2000 2013; 61: 160–176.
5. Gümüş P, Emingil G, Öztürk VÖ, Belibasakis GN, Bostanci N. Oxidative stress markers in saliva and periodontal disease status: modulation during pregnancy and postpartum. BMC Infect Dis 2015; 8: 15:261.
6. Jeffcoat MK, Geurs NC, Reddy MS, Cliver SP, Goldenberg RL, Hauth JC. Periodontal infection and preterm birth: results of a prospective study. J Am Dent Assoc 2001; 132: 875–880.
7. Lopez NJ, Smith PC, Gutierrez J. Higher risk of preterm birth and low birth weight in women with periodontal disease. J Dent Res 2007; 81: 58–63.
8. Buduneli N, Baylas H, Buduneli E, Türkoğlu O, Köse T, Dahlen G. Periodontal infections and preterm low birth weight: a case–control study. J Clin Periodontol 2005; 32: 174–181.
9. Lunardelli AN, Peres MA. Is there an association between periodontal disease, prematurity and low birth weight? A population-based study. J Clin Periodontol 2005; 32: 938– 946.
10. Yücel O, Berker E, Gariboglu S, Otlu H. Interleukin-11, interleukin-1β, interleukin-12 and the pathogenesis of inflammatory periodontal diseases. J Clin Periodontol 2008; 35: 365-370.
11. Carrillo-de-Albornoz A, Figuero E, Herrera D, Bascones-Martínez A. Gingival changes during pregnancy: II. Influence of hormonal variations on the sub-gingival biofilm. J Clin Periodontol 2010; 37(3): 230–40.
12. Van Dyke TE, van Winkelhoff AJ. Infection and inflammatory mechanisms. J Clin Periodontol 2013; 40: 1-7.
13. Offenbacher S, Barros SP, Beck JD. Rethinking periodontal inflammation. J Periodontol. 2008; 79: 1577-1584.
14. Lappin DF, McGregor AMP, Kinane DF. The systemic immune response is more prominent than the mucosal immune response in the pathogenesis of periodontal disease. J Clin Periodontol 2003; 30: 778–786.
15. Kinane DF, Marshall GJ. Periodontal manifestations of systemic disease. Aust Dent J 2001; 46: 2-12.
16. Cutolo M, Seriolo B, Villaggio B, Pizzorni C, Craviotto C, Sulli A. Androgens and Estrogens Modulate the Immune and Inflammatory Responses in Rheumatoid Arthritis. Ann N Y Acad Sci 2002; 966: 131-42.
17. Kovats S. Estrogen receptors regulate innate immune cells and signaling pathways. Cell Immunol 2015; 294(2): 63-69.
18. Cutolo M, S. Capellino, A. Sulli, et al. Estrogen and autoimmune diseases. Ann N Y Acad Sci 2006; 1089: 538–54.
19. Härkönen PL, Väänänen HK. Ann N Y Monocyte–Macrophage System as a Target for Estrogen and Selective Estrogen Receptor Modulators. Acad Sci 2006; 1089: 218-27.
20. Tan IJ, Peeva E, Zandman-Goddard G. Hormonal modulation of the immune system-A spotlight on the role of progestogens. Autoimmun Rev 2015; 14(6): 536-542.
21. Smith JM, Shen Z, Wira CR, Fanger MV, Shen L. Effects of menstrual cycle status and gender on human neutrophil phenotype. The American Journal of Reproductive Immunology 2007; 58: 111–119.
22. Shu L, Guan SM, Fu SM, Guo T, Cao M, Ding Y. Estrogen modulates cytokine expression in human periodontal ligament cells. Journal of Dental Research 2008; 87: 142– 147.
23. Figuero E, Carrillo-De-Albornoz A, Herrera D, Bascones-Martınez A. Gingival changes during pregnancy: I. Influence of hormonal variations on clinical and immunolog- ical parameters. Journal of Clinical Periodontology 2010; 37: 220–229.
24. Bieri RA, Adriaens L, Sporri S, Lang NP, Persson GR. Gingival fluid cytokine expression and subgingival bacterial counts during pregnancy and postpartum: a case series. Clinical Oral Investigations 2013; 17: 19–28.
25. Otenio CC, Fonseca I, Martins MF et al. Expression of IL-1??, IL-6, TNF-??, and iNOS in pregnant women with periodontal disease. Genetics and Molecular Research 2012; 11: 4468–4478.