Detection of Gelatinolytic Activity in Deciduous Sound Dentin

INTRODUCTION: The purpose of present study was to demonstrate the intrinsic gelatinolytic activty in sound dentin of primary teeth by detecting and stimulating the activity of these enzymes.METHODS: Sound dentin from 19 extracted primary teeth were collected and cryo-pulverized into a fine powder. Fluorescein conjugate-gelatin DQ Gelatin EnzCheck gelatinase/collagenase kit (Molecular Probes, Eugene, OR, USA) was used as the cleavage substrate. Intrinsic gelatinolytic enzymes were activated by treating sound dentin powder with 4-Aminophenylmercuric acetate (4-APMA, Sigma-Aldrich International GmbH). A full day (24 h) continuous record of fluorescence emission intensity (FEI) was targeted at 535 nm using 96-well plate reader spectrophotometer (Victor 5 Multilabel Plate Reader, PerkinElmer Life Sciences, Boston, MA, USA).RESULTS: Gelatinolytic activity was detected in sound dentin of primary teeth (981,59±115) and 4-APMA treated dentin exhibited higher gelatinolytic activity (1961,78±204), p

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