Ebru YILMAZ, Elçin BORA, Tufan ÇANKAYA, Ayfer ÜLGENALP, Giray Özlem BOZKAYA, Mahmut ÇOKER, Derya ERÇAL

obez ve dislipidemik Türk çocuklarında apolipoprotein e gen polimorfizmi ve plazma lipid seviyelerinin karşılaştırılması

Amaç: Apolipoprotein E gen polimorfizmi ile lipid metabolizması arasındaki ilişki populasyon çalışmalarında ortaya konulmuştur. Obez olan çocuklarda apolipoprotein E gen polimorfizmi ile plazma lipid düzeyleri arasındaki ilişki ve obezite ile birlikte olan dislipidemilerde apolipoprotein E gen polimorfizmi araştırılmıştır. Yöntemler: İnsülin bağımlı diyabeti, karaciğer ve böbrek yetmezliği bulunmayan, plazma lipid düzeyini etkileyecek ilaç kullanmayan, primer obezitesi bulunan 57 çocuk ve normal kilolu 18 çocuk olmak üzere toplam 75 çocuğun plazma lipid düzeylerine bakılmış ve apolipoprotein E (Apo E) gen polimorfizmi polimeraz zincir reaksiyonu ile analiz edilmiştir. Bulgular: Obez çocuklarda Apo AI düzeyleri yüksek saptanmıştır. Obez ve obez olmayan grupta E2/E3 dağılımı sırasıyla %10,5, %27,8; E3/E3 dağılımı sırasıyla %80,7, %61; E3/E4 dağılımı sırasıyla %7, %5,6 olarak bulunmuştur. Obez olan grupta E4/E4 fenotipi, obez olmayan grupta E2/E4 fenotipi görülmemiştir. Obez olan grupta E2/E4 dağılımı %1,8, obez olmayan grupta E4/E4 fenotip dağılımı %5,6 bulunmuştur. Allel sıklıkları obez ve obez olmayan grupta sırasıyla epsilon 2 (ε2) alleli için %6,1, %14; epsilon3 (ε3) alleli için %89,5, %78; epsilon 4 (ε4) alleli için %4,4, %8 olarak benzer bulunmuştur. Bütün gruplarda en sık allelin ε3 olduğu görülmüştür. Sonuç: Apo E allel ve fenotip dağılımının obez ve sağlıklı çocuklarda benzer olduğu gözlenmiştir. Obez çocuklarda total kolesterol, Apo B, Apo AI ortalama düzeyi, obez olmayan çocuklara göre daha yüksek bulunmuş, trigliserid düzeyleri obez olan grupta obez olmayan gruba göre belirgin yükseklik göstermiştir.

Anahtar Kelimeler:

Apolipoproteinler, Apolipoproteinler E, Çocuk, Lipidler, Obezite, Polimorfizm, genetik, Türkiye, Dislipidemiler

Comparison of apolipoprotein e gene polymorphism and plasma lipid amounts in obese and dislipidemic Turkish children

Objective: The relationship between Apolipoprotein E (Apo E) gene polymorphism and lipid metabolism was presented by the population studies. The aim of this study was to search the relationship between Apo E gene polymorphism and plasma lipid levels in obese children and to search Apo E gene polymorphism in dislipidemias coexisting with obesity.Methods: Seventy five children (57 were primary obese, 18 were normal weight) were analysed by polymerase chain reaction for Apo E gene polymorphism and plasma triglyceride, HDL, LDL and Apo B amounts were evaluated. Excluding criterias for the study group are insulin dependent diabetes mellitus, hepatic and renal failure and medication because of they might affect the lipid concentration in plasma. Results: The Apo AI levels were significantly elevated in obese children. HDL, LDL and Apo B levels were nearly at the same levels while triglycerides levels were much higher in obese group than non-obese group, but the difference was not found statistically significant. In obese and non-obese group the E2/E3 distribution was 10.5% and 27.8%; E3/E3 was 80.7% and 61% and E3/E4 was 7% and 5.6% respectively. The E4/E4 phenotype was not determined in obese group while the E2/E4 phenotype was not seen in non-obese group. E2/E4 distribution was 1.8% in obese children and E4/E4 distribution was 5,6% in non-obese children . Allele frequencies in obese and non-obese children for epsilon 2 (ε2) were found as 6.1% and 14%; for epsilon 3 (ε3) 89.5% and 78% and for epsilon 4 (ε4) 48% and 4% respectively. There was no significant difference between the groups according to allele frequency (p>0.05). The ε3 was found the most frequent allele in all the groups. Conclusion: Apo E allele and phenotype distribution were found similar in obese and non-obese children. The Apo AI levels were significantly elevated in obese children.

Keywords:

Apolipoproteins, Apolipoproteins E, Child, Lipids, Obesity, Polymorphism, Genetic, Turkey, Dyslipidemias,

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