ULAŞ ALABALIK, Hüseyin BÜYÜKBAYRAM, Ayşe Nur KELEŞ, Uğur FIRAT

Helikobakter Pylori Pozitif Gastrit Vakalarında İnflamatuar Hücre Analizi

Amaç: Helikobakter pylori kronik aktif gastrit etiyolojisinde en sık ve en önemli faktör olup, peptik ülser, adenokarsinoma ve MALT Lenfoma gibi çeşitli hastalıklara da zemin hazırlayabilmektedir.Helikobakter pylori gastriti olgularında inflamatuar hücre profilinin ortaya konması Helikobakter pylorinin neden olabileceği hastalıkların patogenezine ışık tutabilmek için önemlidir. Bu çalışmada Helikobakter pylori gastritli olgulardaki inflamatuar hücre profili araştırıldı. Yöntem ve Gereçler: Çalışmaya 100 adet Helikobakter pylori pozitif kronik gastrit ve 20 adet Helikobakter pylori negatif gastrit vakası dahil edildi. Tüm guruplarda H&E ve giemsa boyaması yanında ayrıca immünohistokimyasal olarak CD3, CD4, CD5, CD8, CD10, CD20, CD68, CD79α ve CD138 boyamaları uygulandı ve detaylı inflamatuar hücre analizi yapıldı. Bulgular: İmmünohistokimyasal incelemede, Helikobacter pylori negatif ve pozitif olgular arasında CD3, CD20, CD68 ve CD79α pozitiflikleri açısından anlamlı bir farklılık saptanmadı. Helikobakter pylori miktarı arttıkça CD10 ile ve CD138 ile pozitif reaksiyon veren inflamatuar hücre sayısı anlamlı derecede artarken, CD4, CD5 ve CD8 pozitif olanların sayılarının ise anlamlı derecede azaldığı tesbit edildi. Ayrıca CD138 pozitif plazma hücreleri tüm olgularda en yüksek yoğunluktaki hücre tipiydi. Tartışma ve Sonuç: HP gastritlerinde HP’nin en yoğun olduğu grupta plazma hücrelerinin belirgin biçimde artması ve CD5 pozitif lenfositlerin azalmasının HP ilişkili mide hastalıklarının patogenezinde rol oynuyor olabileceği düşünülmüştür.

Anahtar Kelimeler:

Helikobakter pylori, İnflamasyon, İmmünohistokimya

Analysis Of Inflammatory Cell Profile In Helicobacter Pylori Positive Gastrit Cases

Introduction: Being the most frequent and important factor in the etiology of chronic active gastritis Helicobacter pylori can make up the basis for several diseases like peptic ulcer, adenocarcinoma, and MALT lymphoma. It is important to determined the profile of inflammatory cell in Helicobacter pylori gastritis for lightening the pathogenesis of the diseases the Helicobacter pylori may cause. In this study the inflammatory cell profile of the cases with Helicobacter pylori gastritis was investigated. Methods: 100 cases with Helicobacter pylori positive gastritis and 20 cases with Helicobacter pylori negative gastritis were included in the study. In addition to these CD3, CD4, CD5, CD8, CD10, CD20, CD68, CD79 α and CD138 immunohistochemical stains, H&E and Giemsa stains were performed and detailed inflammatory cell analyse was done.Results: In immunohistochemical investigation, considering the CD3, CD20, CD68 and CD79α positivities, a significant difference was not determined between the groups. It was established that while the number of inflammatory cells positively reacted with CD10 and CD138 was increasing, the number of inflammatory cells positively reacted with CD4, CD5 and CD8 was decreasing significantly with the increasing amounts of Helicobacter pylori. Additionally CD138 positive plasma cells were the most densely seen cell type in all the cases.Discussion and Conclusion: It is considered that in HP gastrits, the obvious increase of plasma cells and the decrease of CD5 positive lymphocytes in the group which has the most HP intensity might be playing a role in HP related stomach disease.

Keywords:

Helicobacter pylori, Inflammation, Immunohistochemistry,

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