Hakan SAĞSÖZ, UĞUR TOPALOĞLU, BERNA GÜNEY SARUHAN, M. Erdem AKBALIK, M. Aydın KETANİ, SEMİH ALTAN, ZEKİ OĞURTAN

Tavşanlarda Asidik Korneal Yanıklarda CD8 ve CD68 Pozitif Hücrelerin Dağılımı

Asitler genel olarak alkali maddelere göre daha az zararlıdır. Temas ettikleri dokularda proteinleri denatüre ederek ve çökerterek hasara neden olurlar. Pıhtılaşmış proteinler de, asidin daha fazla nüfuz etmesini önlemek için bariyer görevi görür. İstisnai bir durum olarak, hidroflorik asitdeki (HFA) floroid iyonlar korneanın derin katmanlarına nüfus eder ve belirgin ön segment yıkımına neden olur. HFA, zaman zaman korneal kök hücre eksikliklerine yol açarak oküler yüzeyde ciddi kronik yangılara neden olabilir. Bu çalışmanın amacı, tavşanlarda HFA ile yakılan korneada CD8 ve CD68 pozitif hücrelerin lokalizasyonunu ve dağılımını incelemektir. Bu amaçla 72 erkek Yeni Zelanda tavşanı kullanıldı. Genel anestezi altında, HFA ile korneal yanık oluşturulduktan sonra dimetil sülfoksit (DMSO), indometazin ve DMSO + indometazin ilaçları ile tedavi uygulandı. Hayvanlar, uygulamaların 2., 7. ve 14. günlerinde ötenazi edildi ve her bir kornea % 10 nötral formol solüsyonunda tespit edildi. CD8 ve CD68 ekspresyonu açısından kontrol, DMSO ve DMSO + indometazin grupları arasında fark yoktu. Ancak, uygulama günleri arasında farklılıklar vardı. Deneylerin 2. ve 7. günlerinde, korneal stromadaki CD8 ve CD68pozitif hücrelerin sayısı artmıştı. Yangı 14. günde azaldığı için indometazin grubu hariç tüm gruplarda CD8 ve CD68pozitif hücrelerin sayısı azalmıştı. Sonuç olarak, bizim bulgularımız gruplar arasında farklılığın olmadığını, CD8 ile CD68 pozitif hücrelerin yangının dönemlerine göre arttığını göstermiştir.

Anahtar Kelimeler:

Asit yanık, CD8, kornea, makrofaj, tavşan

Distribution of CD8 and CD68 Positive Cells in Acid Corneal Burns in Rabbits

Acids are generally less harmful than alkali substances. They cause damage by denaturing and precipitating proteins in the tissues they contact. The coagulated proteins act as a barrier to prevent further penetration. The one exception to this is hydrofluoric acid (HFA), where the fluoride ion rapidly penetrates the thickness of the cornea and causes significant anterior segment destruction. HFA are associated with chronic, severe inflammation of the ocular surface that occasionally lead to corneal stem cell deficiencies. The purpose of this study is to examine the localization and distribution of CD8 and CD68 positive cells in cornea burned with HFA in the rabbit. For this purpose, 72 mature male New Zealand rabbits were used. Under general anesthesia, after a corneal burn was formed by hydrofluoric acid, drug treatments of DMSO, indomethacin and DMSO+indomethacin were performed. The animals were euthanized on the 2nd, 7th and 14th days of the experiment and each cornea was fixed in 10% neutral formol solution. There was no difference among the control, DMSO and DMSO+indomethacin groups in terms of CD8 and CD68 expression. But, there were differences between the days of application. On the 2nd and 7th days of experiments, the number of CD8 and CD68positive cells in the corneal stroma were increased. Because inflammation decreased in 14 days, the numbers of CD8 and CD68 positive cells were decreased in other groups except indomethacin. In conclusion, these findings indicate that there is no difference between the groups and CD8 and CD68 positive cells were increased during the inflammation period.

Keywords:

Acid burn, CD8, cornea, macrophage, rabbit,

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