Uğur UÇAR, MEHMET KÖSE, MEHMET OSMAN ATLI

Konya Merinosu Koyunlarda Gebelik İlişkili Glikoproteinlerin Gebelikteki Plazma Profili ve Erken Gebelik Tanısında Kullanılabilirliği

Gebelik ile ilişkili glikoproteinler (PAGs) koyunları da kapsayan ruminantlarda gebelikte trofoblastik hücrelerden üretilmektedir. Sunulan bu çalışmada Konya Merinosu koyunlarda erken gebelik ve tüm gebelik boyunca kan plazmasındaki PAGs konsantrasyonunun belirlenmesi amaçlandı. Toplam 16 baş gebe koyun kullanıldı. Çalışmanın birinci aşamasında (gebelik boyunca PAGs profilinin izlenmesi) 8 baş koyundan gebeliğin 0, 55, 85, 113 ve 145. günlerinde kan plazması toplandı. İkinci aşamada (erken gebelikteki PAGs profilinin belirlenmesi) ise gebeliğin 18, 21, 25, 28 ve 35. günlerinde kan plazma örnekleri toplandı. Elde edilen plazma örneklerinde PAGs değerleri ticari (Sheep Pregnancy Associated Glycoproteins) ELISA kiti ile ölçüldü. Sonuçlar değerlendirildiğinde gebeliğin 0. gününde plazmada bazal seviyede PAGs olduğu tespit edilmiştir (14,99±1,63 ng/ml). Birinci aşamadaki veriler; gebeliğin 0. günü ile kıyaslandığında; gebeliğin 55, 85, 113 ve 145. günlerindeki değerleri arasında istatistikî olarak önemli bir fark tespit edilmiştir (p<0,05). İkinci aşamada ise gebeliğin 35. gününde plazma PAGs değerinin 18. gün ile kıyaslandığında önemli ölçüde arttığı tespit edilmiştir (p<0,05). Elde edilen sonuçlar değerlendiğinde Konya Merinosu koyunlarda gebelik boyunca plazma PAGs düzeyinin sürekli artan bir profil çizdiği görülmektedir. Yine bu sonuçlara göre çalışmada kullanılan PAGs kiti ile Konya Merinosu koyunlarda en erken 35. günde gebelik teşhisi yapılabileceği söylenebilir.

Anahtar Kelimeler:

Gebelik, Gebelik Teşhisi, Gebelik ile İlişkili Glikoproteinler, Koyun

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