Hasan METİNEREN, Turan Cihan DÜLGEROĞLU, Mehmet Hüseyin METİNEREN

Trombositten zengin Fibrinin periferik sinir iyileşmesi üzerindeki histopatolojik etkileri

Amaç: Sinir iyileşmesini hızlandırmak ve kalitesini artırmak için çeşitli materyallerle deneysel çalışmalar yapılmıştır. Çalışmamızın amacı trombositten zengin fibrinin (TZF) siyatik sinir iyileşmesi üzerindeki histopatolojik etkilerini deneysel rat modelinde araştırmaktır. Yöntemler: 16 Sprague Dawley rat randomize iki guruba (8 kontrol, 8 TZF) ayrıldı. Tüm ratların siyatik sinirleri kesilip 2mm defektli olarak tekrar onarıldı. TZF gurubunda defektif bölgeye TZF sarıldı. 6 haftanın sonunda ratlar sakrifiye edilerek histolojik ve immüno-histokimyasal metotlarla TZF nin kontrol gurubuna göre sinir onarımına katkısı araştırıldı. Sonuç: TZF gurubunun histolojik skorları, kontrol gurubuna göre istatistiksel olarak anlamlı bulundu(P=0.001). TZF sinirlerde aksonal devamlılık mevcuttu. Kontrol grubunda defektin tamamen iyileşmediği yada fibröz/granülasyon dokuyla iyileştiği gözlendi. Bol miktarda hücresel içerik ve büyüme faktörü ihtiva eden TZF sinir iyileşmesine olumlu katkı sağlamaktadır.

Histopathological effects of Platelet rich fibrin on peripheral nerve healing

Objectives: Experimental studies have been carried out with various materials in order to accelerate and improve the quality of nerve healing. Purpose of our study to investigate the histopathological effects of platelet rich fibrin (PRF) on sciatic nerve healing in an experimental rat model. Methods:16 Sprague Dawley rats were randomly divided into the two groups (8 controls, 8 PRF). The sciatic nerves of all rats were transected and repaired with a 2 mm defect. In the PRF group, the defective zone was surrounded with PRF. Both group rats were sacrified and histopathologically examined at the end of 6 weeks. Result: PRF group histological scores were statistically significant (P = 0.001) compared to the control group. Axonal continuity was present in the PRF group nerves. In the control group, improvement of the defect with granulation texture was observed. Conclusion: PRF, which contains abundant amounts of cellular content and growth factors, provides a positive contribution to nerve healing.

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