İsmet DEMİRTAŞ, KUBİLAY DOĞAN KILIÇ, Yiğit UYANIKGİL, Canan YURTTAŞ

Stria longitudinalis medialis ve lateralis’in morfolojik olarak incelenmesi

Amaç: Bu çalışmada, bilinen bazı boyama yöntemlerinin modifiye edilerek stria longitudinalis medialis ve lateralis’in histo-morfolojik olarak incelenmesi ve elde edilecek bulguların bundan sonra yapılacak olan benzer çalışmalara ışık tutması amaçlanmıştır. Yöntemler: Çalışmamızda, %10’luk formaldehit ile tespit edilmiş, makroskobik patolojisi gözlenmeyen, doku bütünlüğü bozulmamış yetişkin kadavra beyin hemisferleri arasından elde edilen dokular kullanıldı. Beş kadavra beyninden alınan örneklerden üçü uygun boyama metoduna ulaşabilmek amacıyla kullanıldı. Doku fiksasyon metodu, Hematoksilen-eozin boyama metodu ve Luxol fast blue boyama metodu uygulandı. Bulgular: Histolojik olarak ilk defa corpus callosum (CC), indusium griseum (IG) ve stria longitudinalis (SL) aynı görüntü üzerinde tespit edildi. SL’in lifleri, vasküler ve morfolojik yapısı belirtildi. Sonuç: Umuyoruz ki, SL ile ilgili histolojik, immünohistokimyasal ve anatomik yaklaşımlar ışığında yaptığımız bu girişim, bu konu üzerindeki bazı karanlık yönleri aydınlatacaktır. Böylece bizden sonra SL üzerinde çalışacak araştırmacıların istifadesine sunulmak üzere akademik bir hafıza oluşturacaktır.

Morphologic study of medial and lateral longitudinal stria

Objectives: The aim of the study is to investigate medial and lateral longitudinal striae histologically and morphologically by some modified staining techniques. The findings of this study may shed some light on future studies. Method: In our study, adult cadaveric brain hemispheres that fixed with 10% formaldehyde solution, without macroscopic pathology and unaffected tissue integrity were used. Three of the samples taken from five cadaver brains were used to reach the proper staining method. Tissue fixation method, Hematoxylin-eosin staining method and the Luxol fast blue staining method was applied. Results: Histologically, corpus callosum (CC), indusium griseum (IG) and longitudinal stria (LS) were detected on the same image for the first time. The fibers and vascular and morphological structures of the LS-ae were indicated. Conclusion: We hope that this initiative in the light of histological, immunohistochemical and anatomic approaches to LS will illuminate some of the dark aspects about this subject. Thus, this will create an academic memory for researchers who studying on LS.

PDF

___

1. Standring S, editor. Gray's anatomy: The anatomical basis of clinical practice, 40th edn. London: Churchill Livingstone Elsevier, 2008.
2. Taner D, editör. Fonksiyonel nöroanatomi, 10. bs. Ankara: Odtü Yayıncılık, 2007.
3. O'Rahilly R, Müller F. The embryonic human brain: An atlas of developmental stages, 3rd edn. USA: John Wiley & Sons, 2006.
4. Shah A, Jhawar SS, Goel A. Analysis of the anatomy of the Papez circuit and adjoining limbic system by fiber dissection techniques. J Clin Neurosci. 2012; 19: 289- 98.
5. Arıncı K, Elhan A. Anatomi, 4. bs. Ankara: Güneş Kitabevi, 2006.
6. Di leva A, Fathalla H, Cusimano MD, Tschabitscher M. The indusium griseum and the longitudinal striae of the corpus callosum. Cortex. 2015; 62: 34-40.
7. Di leva A, Tschabitscher M, Baena RRY. Lancisi's nerves and the seat of the soul. Neurosurgery. 2007; 60: 563-8.
8. Cunningham DJ. Cunningham's textbook of anatomy, 12th edn. New York: W. Wood, 1818.
9. Malobabic S, Puskas L, Vela A. Enlarged medial longitudinal (supracallosal) stria in one human brain. Acta Biol Med Exp. 1988; 13: 87-90.
10. Pavlovic S, Stefanovic N, Malobabic S, et al. Longitudinal striae of the human fornix: shape, relations and variations. Surg Radiol Anat. 2009; 31: 501-6.
11. Swanson LW. Neuroanatomical Terminology: a lexicon of classical origins and historical foundations, 1st edn. USA: Oxford University Press, 2014.
12. Morgane PJ, Galler JR, Mokler DJ. A review of systems and networks of the limbic forebrain/limbic midbrain. Progress in neurobiology. 2005; 75: 143-60
13. Nieuwenhuys R, Voogd J, Van Huijzen C. The human central nervous system: a synopsis and atlas, 4th edn. Berlin Heidelberg New York: Springer Science & Business Media, 2007.
14. Tubbs RS, Prekupec M, Loukas M, Hattab EM, CohenGadol AA. The indusium griseum: anatomic study with potential application to callosotomy. Neurosurgery. 2013; 73: 312-6.
15. Panikkath R, Panikkath D, Mojumder D, Nugent K. The alien hand syndrome. Proc (Baylor Univ Med Cent). 2014; 27: 219–20.
16. Bahuleyan B, Vogel TW, Robinson S, Cohen AR. Endoscopic total corpus callosotomy: cadaveric demonstration of a new approach. Pediatr Neurosurg. 2011; 47: 455-60.
17. Kashem MA, Etages HD, Kopitar-Jerala N, McGregor IS, Matsumoto I. Differential protein expression in the corpus callosum (body) of human alcoholic brain. J Neurochem. 2009; 110: 486-95.
18. Arroyo-Guijarro J, Prats-Galino A, Ruano-Gil D, CostaLlobet C. Comparative study of the medial longitudinal striae of the hippocampus (formerly called “nerves of Lancisi”) in the fighting bull and domestic bull [in French]. Bulletin de l'Association des anatomistes. 1988; 72: 15-9.
19. Rollins NK. Diffusion imaging of the congenitally thickened corpus callosum. AJNR Am J Neuroradiol. 2013; 34: 660-5.
20. Nakada T. High-field, high-resolution MR imaging of the human indusium. AJNR Am J Neuroradiol. 1999; 20: 524-5.
21. Mark LP. Dr. Leighton Mark was invited to comment on this issue. AJNR Am J Neuroradiol. 2000; 21: 982-4.
22. Douet V, Chang L. Fornix as an imaging marker for episodic memory deficits in healthy aging and in various neurological disorders. Front Aging Neurosc. 2014; 6: 343.
23. Catani M, Dell’acqua F, De Schotten MT. A revised limbic system model for memory, emotion and behaviour. Neurosci Biobehav Rev. 2013; 37: 1724-37.
24. Magee KR, Olson RN. The effect of absence of the corpus callosum on the position of the hippocampus and on the formation of Probst's bundle. J Comp Neurol. 1961; 117: 371-82.
25. Schaller K, Cabrilo I. Corpus callosotomy. Acta Neurochir. 2016; 158: 155-60.
26. Kwee RM, Kwee TC. Virchow-Robin spaces at MR imaging. Radiographics. 2007; 27: 1071-86.
27. Biedroń A, Steczkowska M, Kubik A, Kaciński M2. Dilatation of Virchow–Robin spaces in children hospitalized at pediatric neurology department. Neurol Neurochir Pol. 2014; 48: 39-44.