Hasan Hüseyin DÖNMEZ, Fatma ÇOLAKOĞLU, Fatma KILIÇ

Hipotiroidli Gebe Kadınların Periferik Kanında ANAE ve AcP-az Pozitivitesinin Belirlenmesi

Amaç: Gebelik tiroid bezi fonksiyonları için bir stres faktörüdür. En sık görülen tiroid bozukluğu ise hipotiroidizmdir. Bağışıklık sistemi hem gebelikten hem de hipotiroidizmden etkilenmektedir. Bu bozukluk, humoral ve hücre aracılı immün cevaplarda azalmaya neden olmaktadır. Bu çalışma, sağlıklı gebeler ile hipotiroidli gebeler arasındaki alfa naftil asetat esteraz ve asit fosfataz pozitif T lenfosit dağılımlarını karşılaştırmayı amaçlamaktadır. Yöntemler: 2016 Haziran-Aralık ayları arasında sağlıklı ve hipotiroidi olan gebelerden kan örnekleri alındı. Örneklemede 10 sağlıklı gebe olmayan kadın (SK), 10 sağlıklı gebe (SG) ve gebeliğin farklı trimesterlerindeki 30 hipotiroidi gebe (HpGTRI, HpGTRII ve HpGTRIII) kadının kan örnekleri vardı. Bu gruplardaki kişilerin her birinden ANAE ve AcP-az enzim aktivitesi ile May Grünwald-Giemsa için altı adet kan frotisi hazırlanarak histolojik olarak boyandı. Bulgular: En düşük ANAE- ve AcP-az pozitif T lenfosit yüzdeleri sağlıklı gebe grubunda (Grup SG) belirlenirken; bu oranın hipotiroidli gebelerde (HpGTRI, HpGTRII ve HpGTRIII) sağlıklı gebe olmayanlardan (Grup SK) daha yüksek olduğu bulundu (P

Determination of ANAE and AcP-ase Positivity in the Peripheral Blood of Pregnant Women with Hypothyroidism

Objective: Pregnancy is a stress factor for thyroid functions. The most commonly observed thyroid disorder ishypothyroidism. The immune system is affected both by pregnancy and hypothyroidism. This disorder causes adecrease in humoral and cell-mediated immune responses. This study was aimed at the comparison of alpha-naphthylacetate esterase- and acid phosphatase-positive T lymphocyte distribution between healthy and hypothyroidpregnant women.Methods: Blood samples were taken from healthy and hypothyroid pregnant women between June-December 2016.The sampled subjects included 10 healthy non-pregnant women (HC), 10 healthy pregnant women (HP), and 30hypothyroid pregnant women in different trimesters of pregnancy (HpPTRI, HpPTRII and HpPTRIII). From each ofthese groups, six smears were prepared and histologically stained for ANAE and AcP-ase activity and with MayGrünwald-Giemsa.Results: The lowest ANAE- and AcP-ase-positive T lymphocyte percentages were detected in Group HP, and thesepercentages were higher in the hypothyroid pregnant groups, compared to Group HC (P

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