MEHMET CİHAN YAVAŞ, Veysi AKPOLAT, Engin DEVECİ, Hakkı Murat BİLGİN, İbrahim KAPLAN, Uğur ŞEKER, İsmail YILDIZ, Mehmet Esref ALKIŞ, Mustafa Salih ÇELİK, Mehmet Zülküf AKDAĞ

Determining the effect of an electromagnetic field generated by a high voltage power line on rat spermatogonia cells

Objective: Purpose of the study was to research the effect of an electromagnetic field created by a high voltage line onthe testes tissue and the serum biochemistry of Wistar albino male rats. At the same time, in the study also examinedthe protective effects of melatonin and ganoderma lucidum.Methods: In the study, 64 rats were divided into eight equal groups (n: 8). In experiments lasting 26 and 52 days, thefollowing groups were used: High voltage (HV), HV + Ganoderma lucidum (GI), HV + Melatonin (MEL), and Shamcontrol.MEL (10 mg/kg) was administered intraperitoneally and GI (20 mg/kg) as oral lavage. Extremely lowfrequency electric (80.3 V/m) and magnetic fields (2.48 µT) were applied for eight hours per day. Results: The biochemical results of the study show an increase in total oxidant status (TOS) and oxidative stress index(OSI) level and a decrease in total antioxidant status (TAS) level in the 26 and 52 day high voltage groups, comparedto the control group. In the high voltage groups, the nitric oxide (NO) value increased with the increase of theexposure period. Testicular mass (p>0.05), TAS, TOS, OSI and NO (p0.05), but that there was a significant dissimilarity between the 52 day experiment groups(p

Yüksek Gerilim Hattı İle Oluşturulan Elektromanyetik Alanın, Rat Spermatogonium Hücreleri Üzerindeki Etkisinin Belirlenmesi

Giriş: Çalışmanın amacı, yüksek gerilim hattı ile üretilen elektromanyetik alanın testis dokusu ve Wistar albino erkek ratların serum biyokimyası üzerindeki etkisini araştırmaktır. Aynı zamanda çalışmada, melatonin ve ganoderma lucidum' un koruyucu etkilerinin olup olmadığı da incelenmiştir. Yöntemler: Çalışmada, 64 rat sekiz eşit gruba ayrıldı (n: 8). 26 ve 52 gün süren deneylerde, aşağıdaki gruplar kullanıldı: HV, HV+GI, HV+MEL ve kontrol. MEL (10 mg/kg) günlük intraperitonal ve GI (20 mg/kg) oral lavaj olarak uygulandı. Oldukça düşük frekanslı elektrik (80.3 V/m) ve manyetik alanlar (2.48 µT) günde sekiz saat süreyle uygulandı. Bulgular: Çalışmanın biyokimyasal sonuçlarında, kontrol grubuna göre 26 ve 52 günlük yüksek gerilim gruplarında toplam oksidan durum ve oksidatif stres indeksi düzeyindeki artış ve toplam antioksidan durum düzeyinde bir azalma görülmektedir. Yüksek gerilim gruplarında, maruz kalma süresinin artmasıyla nitrik oksit seviyesi artmıştır. 26 günlük karşılaştırmalarda testis ağırlığı (p>0.05), TAS, TOS, OSI ve NO (p0.05), ancak 52 günlük test grupları arasında anlamlı bir farklılık olduğu gösterilmiştir (p

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