Ezgi DOĞANAY YILDIZ, Hakan ARSLAN, Gizem TAŞ, Eyüp Candaş GÜNDOĞDU, Ali KESKIN, Alper YILDIRIM

SODIUM THIOSULPHATE AND TWEEN 80 PREVENT THE FORMATION OF PARA-CHLOROANILINE IN THE IRRIGATING SOLUTIONS

Aim: The aim of the present study was to evaluate the effect of using sodium thiosulphate and Tween 80 to prevent the formation of para-chloroaniline (PCA) between NaOCl and CHX in the apically extruded irrigating solutions. Materials and Methods: Twenty-five extracted human mandibular premolar teeth were instrumented up to size 40. To collect the apically extruded irrigating solution, the roots were placed into the glass vial, and the specimens were divided into 5 groups according to the irrigation protocols, as follows; positive control, negative control, distilled water as intermediate flush between NaOCl and CHX, sodium thiosulphate irrigation between NaOCl and CHX, and Tween 80 irrigation between CHX and NaOCl. The collected liquid was centrifuged and the precipitate was analyzed using 1H NMR spectra. The data were analyzed with chi-square test at 95% confidence level. Results: PCA was detected for all specimens in the positive control group. PCA was absent in the negative control group. Although distilled water irrigation did not prevent the formation of PCA, sodium thiosulphate and Tween 80 prevented the formation of PCA between NaOCl-CHX and CHX-NaOCl, respectively. Conclusion: Within the limitations of the present study, sodium thiosulphate or Tween 80 prevented the formation of PCA between NaOCl-CHX and CHX-NaOCl, respectively. Keywords: chlorhexidine, para chloroaniline, sodium thiosulphate Sodyum Tiyosülftat ve Tween 80 İrrigasyon Solüsyonları İçinde Para-kloroanilin Formasyonunu Engeller Öz Amaç: Bu çalışmanın amacı NaOCl ve CHX arasında sodyum tiyosülfat ve Tween 80 kullanımının apikalden ekstrüze olan irrigasyon solüsyonları içinde PCA formasyonunun önlenmesine etkisinin değerlendirilmesidir. Gereç ve Yöntem: Yirmi-beş çekilmiş mandibular premolar diş 40 numaraya kadar genişletildi. Apikalden ekstrüze olan irrigasyon solüsyonlarının toplanması için, kökler cam şişe içine yerleştirildi, örnekler irrigasyon protokolüne göre 5 gruba ayrıldı: pozitif kontrol, negatif kontrol, NaOCl ve CHX arasında distile su ile yıkama, NaOCl ve CHX arasında sodyum tiyosülfat ile yıkama, CHX ve NaOCl arasında Tween 80 ile yıkama. Toplanan sıvı santrifüje edildi ve çökelti 1H NMR spectra ile analiz edildi. Veriler ki-kare testi ile %95 güven aralığında analiz edildi. Bulgular: Pozitif kontrol grubundaki tüm örneklerde PCA tespit edildi ve negatif gruptaki örneklerin hiçbirinde tespit edilmedi. Distile su irrigayonu PCA oluşumunu önlememesine rağmen, sodium tiyosülfat ve Tween 80 sırasıyla NaOCl -CHX ve CHX- NaOCl arasında kullanımla PCA formasyonunu engelledi. Sonuç: Bu çalışmanın limitasyonları dahilinde, sodium tiyosülfat ve Tween 80, sırasıyla NaOCl -CHX ve CHX- NaOCl arasında kullanımla PCA formasyonunu engellemektedir. Anahtar kelimeler: klorheksidin, para kloroanilin, sodyum tiyosülfat

Anahtar Kelimeler:

chlorhexidine, para chloroaniline

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