ALTERNATİF BİR İRİGASYON SOLUSYONU OLARAK MELATONİNİN SMEAR TABAKASI UZAKLAŞTIRMA ETKİNLİĞİNİN İNCELENMESİ: SEM ÇALIŞMASI

Amaç: Bu çalışmanın amacı, melatoninin kök kanal irigasyon solüsyonu olarak tek başına, % 5.25 sodyum hipoklorit ve % 17 etilendiamintetraasetikasit ile birlikte kullanımının smear tabakasını kaldırma etkisini incelemektir.Gereç ve Yöntem: Toplam 75 adet yeni çekilmiş, tek köklü, insan maksiller santral kesici dişi bu çalışma için seçildi. Dişler kuronlarından keserek ayrıldıktan sonra kalan kökler irigasyon protokollerine göre (n = 15) beş gruba ayrıldı: Grup 1: % 5.25 sodyum hipoklorit; Grup 2: % 0.02 melatonin Grup 3: % 5.25 sodyum hipoklorit + % 17 etilendiamintetraasetikasit; Grup 3: % 5.25 sodyum hipoklorit + % 0.02 melatonin Grup 5: % 0.02 melatonin + % 17 etilendiamintetraasetikasit. Kökler uzun eksenleri boyunca iki yarım parçaya ayrıldı. Bu parçalar üzerinde smear tabakasının varlığı; şayet var ise miktarı taramalı elektron mikroskobu kullanılarak incelendi ve skorlandı. Elde edilen veriler SPSS programında Kruskal-Wallis testi kullanılarak istatistiksel olarak analiz edildi (α =0.05).Bulgular: Melatonin, sodyum hipoklorit ile kıyaslandığında kök kanalının koronal ve orta üçlü bölgesinde smear kaldırma kapasitesi anlamlı derecede yüksek bulundu (p<0.05). Sodyum hipoklorit + etilendiamintetraasetikasit kullanımı ile melatonin + etilendiamintetraasetikasit kullanımı koronal üçlüde smear kaldırma etkisi açısından anlamlı bir fark göstermedi (p>0.05). Apikal bölgede grupların smear kaldırma etkinlikleri değerlendirildiğinde sodyum hipoklorit + etilendiamintetraasetikasit en iyi smear kaldırma kapasitesine sahip bulundu. (p<0.05)Sonuç: Bu çalışmanın sınırları dahilinde, 0.2 mg / mL melatonin 1 dakika boyunca irigant olarak kullanımı smear tabakasının kaldırılmasında anlamlı derecede etkili bulunmuştur. Melatoninin konsantrasyonunun, uygulama süresinin veya miktarının smear tabakasını kaldırma kapasitesini nasıl etkileyeceğini belirlemek için daha fazla araştırmaya ihtiyaç vardır.Anahtar Kelimeler: Melatonin, Smear, Taramalı Elektron Mikroskobu. INVESTIGATION OF SMEAR LAYER REMOVAL EFFECTIVENESS OF MELATONIN AS AN ALTERNATIVE IRRIGATION SOLUTION: SEM STUDYAbstractAim: The aim of this study was to investigate the smear layer removal effectiveness of melatonin which was used alone, together with 5.25% sodium hypochlorite and with 17% ethylenediaminetetraaceticacid as a root canal irrigation solution.Materials and Methods: A total of 75 freshly-extracted, single-rooted, human maxillary central incisors were selected for this study. Following decoronation of teeth, remaining root parts were subdivided into five groups according to irrigation protocols groups (n = 15): Group 1: 5.25 % sodium hypochlorite; Group 2: 0.02 % melatonin; Group 3: 5.25 % sodium hypochlorite + 17 % ethylenediaminetetraaceticacid; Grup 4: 5.25 % sodium hypochlorite + 0.02 % melatonin; Grup 5: 0.02 % melatonin + 17 % ethylenediaminetetraaceticacid. All the roots were longitudinally split into two halves. Presence of smear layer, if present the amount of it was examined and scored on these half parts by using scanning electron microscopy. The data were analyzed statistically by using Kruskal-Wallis test in SPSS program (α = 0.05).Results: When melatonin was compared to sodium hypochlorite, smear removal capacity was significantly higher in coronal and middle one–third region of the root canal (p <0.05). No significant difference was observed between the use of sodium hypochlorite + ethylenediaminetetraaceticacid and melatonin + ethylenediaminetetraaceticacid in terms of smear removal capacity on coronal one–third region (p> 0.05). When smear removal effect of the groups were evaluated, sodium hypochlorite + ethylenediaminetetraaceticacid was found to have the best smear removal capacity in the apical one-third region (P <0.05).Conclusions: Within the limits of this study, the use of 0.2 mg / mL melatonin as an irigant for 1 minutes application was found to be significantly effective in removing the smear layer. Further research is needed to determine how the concentration, amount or duration of melatonin will affect the capacity to remove the smear layer. Key word: melatonin, smear, scanning electron microscopy.

Anahtar Kelimeler:

Melatonin, Smear

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