Serpil DEĞERLİ, Zeynep SÜMER, Semra ÖZÇELİK

The scolocidal effect of propolis on pro- toscoleces and daugther cysts

Amaç: Günümüzde skolosidal ajanlar hidatik kist sıvısının tedavi sırasında kistin dışınadağılmasını önlemek amacıyla kullanılmaktadır. Etkili bir skolosidin protoskoleksler üzerindekietkisinin yanı sıra, toksik olmayan, kolay uygulanabilen sklerozan kolanjit oluşturmayan ve kızveziküllerin de içine nüfuz edebilen özelliklere sahip olması beklenmektedir. Bu çalışmada doğalbir arı ürünü olan propolisin skolosidal etkisinin araştırılması amaçlanmıştır.Yöntem: Çalışmada, karaciğer hidatik kisti bulunan bir hastadan operasyon sırasında elde edilen,kız veziküller ve protoskoleksler ve Karadeniz bölgesinde elde edilen propolis kullanılmıştır.Canlılık tesbiti %0,1lik Eosin solüsyonu kullanılarak yapılmıştır. Propolis DMSO içindeçözülerek, %0,9luk serum fizyolojik içinde 50 mg/mL ile 0,010 mg/mL arasında sulandırılmıştır(4000 protoskoleks/mL) ve tüplere 100er µl dağıtılmıştır. Farklı konsantrasyonlardaki propolisin1.,3.,5.,10.,20.,ve 30. dakikalarda protoskoleksler üzerine etkisi hemositometre lamında sayımyapılarak belirlenmiştir. Ayrıca, 4 ila7 mm boyutlarında olan çok sayıdaki kız veziküllere 0,80 ve3,12 mg/mLde hazırlanan propolis uygulanmış,5.,10.,20.,30., dakikalarda kistler açılarakiçlerindeki protoskolekslerin canlılık durumları araştırılmıştır.Bulgular: Propolisin 0,100 mg/mLde ilk dakikada, 0,050 mg/mLde 3. dakikada, 0,025mg/mLde 10. dakikada, 0,010 mg/mLde 20. dakikada protoskoleksler üzerine öldürücü etkisigözlenmiştir. Kız veziküller içindeki protokoleksler 0,80 mg/mLlik konsantrasyonda 20. dakikadaprotoskolekslerin %50si, 3,12 mg/mLlik konsantrasyonda 10. dakikada ise tamamı etkilenmiştir.Sonuç: Özellikle, 10 dakika gibi kısa bir sürede kız veziküllere de etki edebilen doğal maddepropolisin skolosidal ajan olarak kullanılabileceği ve bu konuda daha ileri çalışmalara gereksinimolduğu kanısına varılmıştır.

Propolisin protoskoleksler ve kız kistler üzerine skolosidal etkisi

Objective: Scolocidal elements are used in the aim of not scattering the protoscoleces outside thecyst during the treatment of cyst hydatid in our present-day. It is hoped from the scolocidalmaterials; besides its effect on protoscoleces, not being toxic, being easy practicable, not causingsclerozan cholanjit and if possible, penetrating in the daughter vesicules. In this research, the effectof propolis which is a bee product on protoscoleces were investigated.Methods: In our work, daughter vesicules and protoscoleces obtain from the liver of a patientduring surgery and propolis from the Black Sea Region were used. Viability tests were done byusing 0.1% eosine solution. After propolis disolved in dimethylsulfoxide, within the range of 50mg/mL and 0.010 mg/mL using by 0.9% serum physiological solution 100 µl into each of the tubeand in 1.,3.,5.,10.,20.,and 30. minutes the rates of viability of protoscolex (4000 protoscolex/mL)are fixed. The research were subjected to daughter vesicules that is 4-7 mm in diameters. Theconcentration of propolis at 0.80 and 3.12 mg/mL were investigated viability rates of protoscolexat different incubation time and whether the possibilty of penetration to the daughter vesicules.Results: It was seen that protoscolexs were effected by the Propolis at 0.100 mg/mL concentrationin the first minute, at 0.050 mg/mL concentration in the 3. minutes, at 0.025 mg/mL concentrationat 10. minutes and at 0.010 mg/mL concentration in 20. minutes. For protoscolex in the vesiculesat 0.80 mg/mL concentration in 20. minute, in 3.12 mg/mL concentration in 10. minutes werefound to be lethal.Conclusions: As the protoscolex could be killed by Propolis which penetrate to daughter cysts in avery short time as 10 minutes, we thing that it will be beneficial completing advanced studies.

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