Gözde ŞAHİN, Atmaca Şahin SAĞALTICI, Şefik GÜRAN, Zehra Dilşad ÇOBAN, Orhan FERMANLI

NGN 3 and Klf 4 have important role in fetal mouse brain development

Amaç: Nörogenez ve gliogenezden oluşan embriyonal beyin gelişimi farklı aşamalarda çeşitligenlerin katımlı ile düzenlenen bir süreçtir. Nöronal ve glial doku gelişiminde bazıtranskripsiyonal faktörlerin rolü olduğu bilinmektedir. Ancak bu transkripsiyonal faktörlerin beyingelişiminde hamileliğin farklı dönemlerindeki rolleri tam olarak açık değildir.Yöntem: Çalışmamızda fare fetal beyin dokularında NGN 3, Klf 4, c-Myc, Nanog, Oct ¾, GATA4, Sox 2 transkripsiyonal faktör genlerinin ekspresyon düzeylerine hamileliğin birinci ve üçüncütrimester sonlarına da dönemlerde bakılmıştır.Bulgular: Elde edilen sonuçlar erişkin fare beyninden elde edilen sonuçlarla karşılaştırılmıştır.Birinci trimester sonunda NGN 3, Klf 4, cMyc, Nanog, Oct ¾ genlerinde kontrole göre yüksek genekspresyon düzeyleri saptanırken, GATA 4 geninde kontrole göre benzer gen ekspresyon düzeyibulunmuştur. Sox 2 geninde ise çok düşük gen ekspresyon düzeyi tespit edilmiştir. Her gene içinen fazla ekspresyon düzeyi ilk trimesterda bulunmuştur. Bu genlere ait ekspresyon düzeylerihamilelik süresince zamana bağlı olarak azalmıştır. İlginç olarak bizim seride NGN 3 ve Klf 4 genekspresyon düzeyleri kontrol gen düzeyine göre birinci ve üçüncü trimetserde oldukça yüksektir.Sonuç: NGN 3 ve Klf 4 genlerinin hamilelik süresince fetal fare beyninin gelişimindeki önemlirole sahiptir.

NGN 3 ve Klf 4 fetal dönem fare beyni gelişiminde önemli role sahiptir

Objective: Embryonal brain development including neurogenesis and gliogenesis requires theconcerted actions of numerous genes that are regulated at multiple levels. As known, transcriptionfactors have roles in neural and glial development. But the roles of these factors in braindevelopment in different gestational periods are not clear.Method: The gene expression profiles of NGN 3, Klf 4, c-Myc, Nanog, Oct ¾, GATA 4, Sox 2genes had been searched in brain tissues of mice fetus at the end of the first and the thirdtrimesters.Results: The results were correlated with the results obtained from brain tissue of adult mouse. Atthe end of the first trimester, higher gene expression levels were found due to internal control inNGN 3, Klf 4, c-Myc, Nanog, Oct ¾. Similar gene expression levels were observed in GATA 4.Dramatically lower gene expressions were analyzed in Sox 2. In each gene, the highest geneexpression levels were found in the first trimester. The expression level of each gene wasdecreased due to time period during the pregnancy. Interestingly, the gene expression levels ofNGN 3 and Klf 4 were extremely high due to control gene level in the first and third trimesters inour series.Conclusion: According to these results, NGN 3 and Klf 4 genes have important roles in fetal micebrain development during the gestation period.

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