Effect of beta-glucan in preventing bacterial translocation in a model of experimental obstructive jaundice

Amaç. Sepsis, barsaktan kaynaklanan bakteriyel translokasyonun bir sonucu olarak tıkanmasarılığında post-operatif morbidite ve mortalitenin en önemli sebebidir. Bu çalışma, ortak safrakanalı ligasyonu yoluyla tıkanma sarılığı geliştirilen bir hayvan modelinde bakteriyeltranslokasyonun önlenmesinde beta-glukanın etkisi araştırmak için yapılmıştir. Yöntem. Kırk beşadet Wister albino rat, her birinde onbeş hayvanın bulunduğu üç gruba ayrıldı. I.gruba sadecelaparatomi yapıldı. II. gruba safra kanalı ligasyonu yapıldı. III. gruba ise safra kanalı ligasyonu veon gün beta-glukan oral verildi. Onuncu günün sonunda hayvanlar sakrifiye edildi. Kan, karaciğer,dalak ve mezenter lenf nodu kültürleri ekildi. Terminal ileum ve karaciğerden alınan örneklerhistopatolojik olarak incelendi. Ratlardan alınan kan örneklerinden aspartate aminotransferaz(AST), alanine aminotransferaz (ALT), alkalin fosfatase (ALP), laktat dehidrogenaz (LDH), totalbilirubin, direk Bilirubin ve C-reaktif protein (CRP) çalışıldı. Bulgular. Birincigrupta 1 hayvandabakteriyel translokasyon gözlenirken, II. grupta bakteriyel translokasyon oniki hayvanda gözlendi.III. grupta beta-glukan verilen hayvanların altı tanesinde bakteriyel translokasyon tespit edildi. Buüç grup arasındaki fark istatistiki açıdan anlamlıydı (p

Deneysel tıkanma sarılığı modelinde beta-glukanın bakteriyal translokasyonu önlemedeki etkinliği

Aim. Sepsis is the major cause of post-operative morbidity and mortality in obstructive jaundice asa result of bacterial translocation from the gut. This study was conducted to investigate the effectof beta-glucan in preventing bacterial translocation in an animal model where obstructive jaundicewas developed by common bile duct ligation. Methods. Forty-five Wistar-albino rats were dividedinto three groups of fifteen animal each. Only laparotomy was administered to the first group. Bileduct ligation was administered to the second group. Bile duct ligation and oral beta-glucan for tendays were administered to the third group. The animals were sacrified at the end of the tenth day.Blood, liver, spleen and mesenteric lymph nodes were cultured. The samples taken from terminalileum and liver were examined histopathologically. Aspartate transaminase (AST), alaninetransaminase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), total bilirubin,direct bilirubin and C-reactive protein (CRP) analyses were done on the blood samples taken fromthe rats. Results. In the first group bacterial translocation was observed in one animal whereasbacterial translocation was observed in twelve animals in II nd group. In third group bacterialtranslocation was determined in six animals that received beta-glucan. The differences betweenthese three groups were statistically significant (p

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