Ali ÇETİN, Ahmet ALTUN, Barış KARADAŞ, Ali KULA, İhsan BAĞCİVAN, Nedim DURMU, Tijen Kaya TEMİZ

Analysis of the antinociceptive effect of pethidine combination with ketamine or paracetamol in tail-flick test in mice

Amaç. Petidin gibi opioid ilaçlar, ciddi akut ve kronik ağrı tedavisinde kullanılan en etkili analjezik ilaçlardır. Bu araştırmada fare tail-flick testi ile ketamin veya parasetamol ile petidinin potansiyel antinosiseptif ilişkisi incelendi. Yöntem. Elli erkek BAL/c fare rastgele olarak beş gruba ayrıldı. Üç grupta intraperitoneal petidin (10-100 mg/kg), ketamin (1.25-5 mg/kg) ya da parasetamolün (2.5-10 mg/kg) antinosiseptif etki süresi tail flick latens (TFL) süreleri ölçülerek incelendi. Diğer iki grupta ketamine (2.5 mg/kg) veya parasetamol (1.25 mg/kg) ile birlikte petidinin (10 mg/kg) minimal etkin dozunun kombinasyonu TFL ile değerlendirildi. Bulgular. İntraperitoneal olarak uygulanan petidin doz bağımlı olarak 10-50 mg/kg'dan başlayarak ağrı kesici etki sağladı, fakat kullanılan dozlarda diğer ilaçlar etkisizdi. Ketamin (2.5 mg/kg) veya parasetamolün (1.25 mg/kg) bu etkisiz dozları, petidin ile kombine edildiğinde antinosiseptif etkiyi anlamlı olarak güçlendirdi (p

Fare tail-flick testi ile ketamin veya parasetamol ile birlikte kullanılan petidinin antinosiseptif etkisinin incelenmesi

Aim. The opioid drugs, such as pethidine, are the most effective analgesic drugs used in the treatment of severe acute and chronic pain. In this study, we aimed to investigate the potential antinociceptive interaction of pethidine with ketamine or paracetamol in tail-flick test in mice. Methods. Fifty male BALB/c mice were divided randomly into 5 groups. In three of the groups, the time course of antinociceptive action was determined after using intraperitoneal pethidine (10-100 mg/kg), ketamine (1.25-5 mg/kg) or paracetamol (2.5-10 mg/kg) assessing tail flick latencies (TFLs). In the remaining two groups, combination of minimal effective dose of pethidine (10 mg/kg) with ketamine (2.5 mg/kg) or paracetamol (1.25 mg/kg) were assessed by TFLs. Results. Intraperitoneally administered pethidine revealed a dose dependent antinociception at 10-50 mg/kg doses but ketamine and paracetamol were ineffective at used doses. These ineffective doses of ketamine (2.5 mg/kg) or paracetamol (1.25 mg/kg) significantly potentiated the antinociceptive effect when combined with pethidine (p

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