Ecehan EFE, Emine YALÇIN, Kültiğin ÇAVUŞOĞLU

Smilax excelsa L. Meyve Ekstresinin Antimutagenik ve Multi-Biyolojik aktiviteleri

Bu çalışmada, Smilax excelsa L. meyve ektraktının antimikrobiyal, antioksidan ve antimutajenik aktiviteleri araştırılmıştır. Antimikrobiyal aktivite disk difüzyon yöntemi ile antimutajenik aktivite ise Ames/Salmonella/mikrozom testi kullanılarak araştırılmıştır. S. excelsa meyve örneklerinin antioksidan özelliği ise toplam fenolik ve flavonoid içeriğinin tespiti ve 1,1-difenil-2-pikrilhidrazil (DPPH) giderme etkisi araştırılarak belirlenmiştir. Meyve ekstraktının 200 mg plak-1 dozunda Salmonella typhimirium TA1535 suşu ile S9 karışımı yokluğunda %81, S9 karışımı varlığında ise %67 oranında mutajenite inhibisyonu oluşturduğu belirlenmiştir. S. excelsa meyve eksraktının test edilen mikroorganizmalara karşı 11-16 cm aralığında inhibisyon zonu oluşturduğu gözlenmiştir. 200 mg mL-1 konsantrasyonunda ekstraktta flavonoid içeriği 0.7985±0.0124 mgQE 100 mL-1 olarak, fenolik içerik ise 11.9847±0.0041 mgGAE 100 mL-1 olarak tespit edilmiştir. 200 mg L-1 dozunda DPPH giderme oranı %55 olarak belirlenmiştir. Sonuç olarak, S. excelsa meyve dokularının yüksek antimutajenik aktivite sergilediği, potansiyel doğal bir antimutajenik ve antioksidan kaynak olduğu olduğu belirlenmiştir.

Antimutagenic and Multi-Biological Activities of Smilax excelsa L. Fruit Extract

In this study, antimutagenic, antimicrobial and antioxidant activities of Smilax excelsa L. fruit extract were evaluated. The antimicrobial effect was investigated by disk diffusion method and the antimutagenic effect was investigated by Ames/Salmonella/microsom test. The antioxidant properties of S. excelsa fruit samples were determined by investigating the total phenolic, flavonoid contents and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. It was observed that 200 mg plate-1 fruit extract was found to have a mutagenicity inhibition as 81% in the absence of S9 mixture and 67% in the presence of S9 mixture against Salmonella typhimurium TA1535. S. excelsa fruit extract produced an inhibition zone in the range of 11-16 cm against the tested microorganisms. Flavonoid and phenolic contents were found to be 0.7985±0.0124 mgQE 100 mL-1 and 11.9847±0.0041 mgGAE 100 mL-1 at the 200 mg mL-1 extract concentration, respectively. The DPPH removal rate was determined to be 55% at the 200 mg L-1 dose. As a result, it was observed that the S. excelsa fruit tissues exhibited a highly antimutagenic activity and has been determined as a potential natural antimicrobial and antioxidant source.

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