Proteinleri Ayırmak İçin Ters Faz Kromatografisi (RPC) Kolon Dolgu Materyali Tasarımı: Bölüm 1- Sentez ve Karakterizasyon

İki bölümden oluşan çalışmanın ilk bölümünde Ters Faz Kromatografisi (Reversed Phase Chromatography, RPC) için eş boyutlu ve gözenekli partikül formunda poli(vinil asetat-co-divinilbenzen) [poli(VAc-co-DVB)] ve poli(divinilbenzen) [poli(DVB)] kolon dolgu materyalleri "Çok Basamaklı Mikrosüspansiyon Polimerizasyonu" yöntemi ile sentezlenmiştir. Partiküller bazik ortamda hidrolizlenerek, partikül yüzeyi "hidroksil" formuna dönüştürülmüş ve kromatografik kullanım için uygun yüzey ve gözenek yapısına sahip poli(vinilalkol-co-divinilbenzen) [poli(VA-co-DVB)] partiküller elde edilmiştir. Partiküllerin karakterizasyonu Taramalı Elektron Mikroskobu (Scanning Electron Microscopy, SEM), yüzey alanı ve gözeneklilik ölçüm sistemi (Brunauer-Emmett-Teller, BET) ve Fourier Transfer Infrared Spektrofotometre (Fourier Transform Infrared Spectroscopy, FTIR) ile yapılmıştır

Design of Reversed Phase Chromatography (RPC) Column Packing Material for Protein Separation: Part 1- Synthesis and Characterization

In the first part of the two-part study, the column packed materials in the form of monodisperse and porous particles were synthesized with regard to test their column performances in Reverse Phase Chromatography (RPC). Particles in the form of poly(vinyl acetate-co-divinylbenzene) [poly(VAc-co-DVB)] and poly(divinylbenzene) [poly(DVB)] were produced by “multi-stage micro-suspension polymerization”. The surface of particles were converted to “hydroxyl” form by base hydrolysis that were used as poly(vinylalcohol-co-divinylbenzene) [poly(VA-co-DVB)] particles, having appropriate structural properties like surface and porosity for chromatographic applications. Particle characterization was assessed by Scanning Electron Microscopy (SEM), BET surface area and porosity measurement system and Fourier Transform Infrared Spectroscopy (FTIR)

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