Poli(Hidroksietilmetakrilat-Genişletilmiş Perlit) Kompoziti ile Yumurta Akından Lizozimin Tek Basamakta Saflaştırılması

Bu çalışmada poli(hidroksietilmetakrilat-genişletilmiş perlit) P(Hema-Ep) kompozit materyali kullanılarak sulu çözeltiden lizozim adsorpsiyonu çalışıldı. Bu amaçla öncelikle P(Hema-Ep) kompoziti sentezlendi ve SEM, FTIR ve şişme testleri ile karakterizasyonu yapıldı. İsoelektrik noktası 10.7 (pI) ve molekül kütlesi 14 kDa olan tavuk yumurta beyazı lizozimi, enzimi model protein olarak kullanıldı. Oda sıcaklığında ve 0.1 M pH:8.0 fosfat tamponunda gerçekleştirilen deney sonucunda adsorpsiyon kapasitesi 51 mg g-1 olarak bulundu. Desorpsiyon ve tekrar kullanılabilirlik deneyleri yapıldı. Sentezlenen P(Hema-Ep) kompoziti verimini kaybetmeden on kere tekrar kullanılabilir olduğu saptandı. Sentezlenen bu yeni kompozit ile lizozim enzimi tek basamaka saflaştırıldı.

New Composite, Poly(Hydroxyethylmethacrylate-Expanded Perlite), for Single step Separation of Egg White Lysozyme

In this study, we investigated adsorption behaviour of lysozyme on poly(hydroxyethylmethacrylateexpanded perlite) [P(Hema-Ep)]. For this purpose, we synthesized P(Hema-Ep) composite by bulk polymerization and it was characterized by SEM, FTIR and swelling ratio. The fresh chicken egg white lysozyme was used as a model protein, had an isoelectric point (pI) 10.7 and molecular weight (MW) 14 kDa. Adsorption capacity was found 51 mg g-1 at room temperature and in 0.1 M pH=8.0 phosphate buffer. Experiments on desorption and reusability were also performed. It appears that P(Hema-Ep) composites can be used ten times without lost of efficiency. Lysozyme purification, without causing any denaturation, can be performed from chicken egg white by using this new synthesized composite in a single step.

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