Ceylan HEPOKUR, Hasan Bahri KARAYEL, Bedrettin SELVİ, Yakup BUDAK, İlhan YAYLIM

Inula Viscosa Ekstraktının Sitotoksik Etkilerinin Araştırılması

Inula viscosa farklı hastalıkların tedavisinde medikal amaçlı kullanılan Asteraceae ailesine ait bir türdür. Inula viscosa monoterpene, seskiterpen, diterpen ve flavonoid gibi pek çok biyolojik bileşen içerir. Çalışmamızda Inula viscosa ekstraktlarının MCF-7, C6, MG63 ve L929 hücre hatlarında sitotoksik etkisinin incelenmesini amaçladık.Inula viscosa ekstraklarının sitotoksik etkisi XTT analizi ile yapıldı.Bu çalışmada Inula viscosa ekstrakları MCF-7 hücre hatlarında L929’a göre seçici sitotoksik etki gösterdi. Inula viscosa ekstrakları, ümit verici bir yeni antikanser ajan kaynağı olarak görünmektedir. Bu kanser hücre hatları üzerindeki sitotoksik aktivite mekanizmalarını tanımlamak için daha ileri araştırmalara ihtiyaç vardır.

Investigation of Cytotoxic Effects of Inula Viscosa Extract

Inula viscosa is a type of Asteraceae family, which widely used for medicinal plant to treatment ofdifferent diseases. Inula viscosa contains many biological compounds, such as monoterpenes, sesquiterpenes,diterpenes, flavonoids. Our study aimed to investigate the cytotoxic effects of Inula viscosa extract on MCF-7(breast carcinoma), C6 (glioblostoma cancer), MG63 (bone osteosarcoma), cancer cells and L929 (mousefibroblostama) cell lines. Cytotoxic effects of the Inula viscosa extracts were performed by XTT assay. In thisstudy, Inula viscosa extracts showed selective cytotoxic effect on MC-7 compared than L929 cells. The Inulaviscosa extracts appear to be a promising source of new anticancer agent. Further studies are needed toidentify the cytotoxic activity mechanisms on these cancer cell lines.

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Basanagouda M., Jambagi V.B., Barigidad N.N., Laxmeshwar S.S., Devaru V. and Narayanachar M., Synthesis, Structure-activity relationship of iodinated-4-aryloxymethylcoumarins as potential anti-cancer and antimycobacterial agents, European Journal of Medicinal Chemistry, 74 (2014) 225-233.
Barrett J.M., Mechanisms of Multistep Carcinogenesis and Carcinogen Risk., Environmental Health Perspectives,100 (1993) 9-20.
Tan W., Lu J., Huang M., Li Y., Chen M., Wu G., Gong J., Zhong Z., Xu Z., Dang Y., Guo J., Chen X. and Wang Y., Anti-cancer natural products isolated from Chinese medicinal herbs. Chinese Medicine,6 (2011) 27- 42.
Monteiro L.S., Bastos K.X., Barbosa-Filho J.M., Athayde-Filho P.F., Diniz M.F. and Sobral M.V., Medicinal Plants and Other Living Organisms with Antitumor Potential against Lung Cancer., Evidence-Based Complementary and Alternative Medicine, Article ID 604152 (2014) pp 15.
Brullo S. and Marco G., Taxonomical revision of the genus Dittrichia (Asteraceae), Porttugaliae Actua Biol,19 (2000) 341-54.
Messaoudi M., Chahmi N., El Mzibri M., Gmouh S., Amzazi S., Benbacer L. and El Hassouni M., Cytotoxic Effect and Chemical Composition of Inula viscosa from Three Different Regions of Morocco., European Journal of Medicinal Plants, 16-4 (2016) 1-9.
Ana M.L.S., Grigore A., Diana C.G.A.P. and Artur M.S.S., The genus Inula and their metabolites: From ethnopharmacological tomedicinaluses., Journal of Ethnopharmacology, 154 (2014) 286-310.
Su B.N.,Takaishi Y., Yabuuchi T., Kusumi T., Tori M., Takaoka S., Honda G., Ito M., Takeda Y., Kodzhimatov O. K. and Ashurmetov O. Sesquiterpenes and monoterpenes from the bark of Inula macrophylla., J. Nat. Prod., 64 (2001) 466.
Barbetti P., Chiappini I., Fardella G. and Menghini A., A new eudesmane acid from Dittrichia (Inula) viscosa., Planta Med., 51-5 (1985) 471.
Lauro L. and Rolih C. Observations and research on an extract of Inula viscose Ait. Boll, Soc. Ital. Biol. Sper.,66 (1990) 829-834.
Lev E. and Amar Z. Ethnopharmacological survey of traditional drugs sold in Israel at the end of the 20th century., J. Ethnopharmacol,72 (2000) 191-205.
Zhaoa Y., Zhanga M., Shi Q. and Kiyota H., Chemical Constituents of Plants from the Genus Inula., Chemıstry & Biodiversity, 3 (2006) 371.
Adzu B., Balogun O.S., Sérgio E.D., Donizeti A.I., Mendes S.R., Wagner S.A., Reginaldo V.R.Â., Márcia Selhorst S.B.R., Godinhode O.L., Ireneda S.A., Sabino D.D. and Tabajara de O.M., Evaluation of the safety, gastroprotective activity and mechanism of action of standardised leaves infusion extract of Copaifera malmei Harms., Journal of Ethnopharmacology, 175 (2015) 378-389.
Tanaka R., Shıma K., Taruı S. and Nıshıkawa M., Boiling Method for the Extraction of Gut Glucagon-like Immunoreactive Materials, Endocrinol. Japon, 22-6 (1975) 503-507.
Tutar U., Celik C., Karaman I., Hepokur C. and Atas M. The anti-biofilm and antimicrobial activity of Mentha pulegium L. essential oil against multidrug-resistant Acinetobacter baumannii. Trop J Pharm Res., 15-5 (2016) 1039-1046.
Lastra C., Lopez A. and Motilva V., Gastroprotection and prostaglandin E2 generation in rats by flavonoids of Dittrichia viscosa. Planta Med., 59-6 (1993) 497–501.
Haoui IE., Extraction of Essential Oil from Inula viscosa (L.) Leaves: Composition, Antifungal Activity and Kinetic Data. Journal of essential oil-bearing plants, 19-1 (2016)108- 118.
Rozenblat S., Grossman S., Bergman M., Gottlieb H., Cohen Y. and Dovrat S., Induction of G2/M arrest and apoptosis by sesquiterpene lactones in human melanoma cell lines, Biochemical Pharmacology, 75 (2008) 369-82.
El Ouariachi E. and Bouyanzer A., Antioxidant activity of solvent extracts of Inula viscosa from Morocco, Arabian Journal of Chemical and Environmental Research, 1(2014)33-40.
Seca A.M., Grigore A., Pinto D.C. and Silva A.M., The genus Inula and their metabolites:From ethnopharmacological to medicinal uses., J Ethnopharmacol,154-2 (2014) 286-310.
Belayachi L., Aceves-Luquero C., Merghoub N., Bakri Y., Fernández de Mattos S., Amzazi S. and Villalonga P., Screening of North African Medicinal Plant Extracts for Cytotoxic Activity Against Tumor Cell Lines., European Journal of Medicinal Plants,3-3 (2013) 310- 332.
Dorn D.C., Alexenizer M. and Hengstler J.G. Dorn A., Tumor cell specific toxicity of Inula helenium extracts, Phytother Res., 20- 11(2006)970-80.