Comparing the antimicrobial effects of silver and copper nanoparticles against pathogenic and resistant bacteria of Klebsiella pneumonia, Pseudomonas aeruginosa and Staphylococcus aureus

Abstract. Topics and Objectives: In this study, the effectiveness of antimicrobial activity of Ag and Cu nanoparticles against Gram positive bacteria and Gram-negative resistant pathogens and common nosocomial infections were observed.Methods: At first, in order for the effect of different concentrations of nanoparticles on bacteria in macrodilution method of 0.01, 0.1, 0.5, 1, 1.5 % of silver and copper nanoparticles (medium + nanoparticles) were prepared and added to 105 cell/ml of each of the bacteria.Dishes containing the treated cultures (bacteria + nanoparticles) and control media were placed in a shaking incubator. Then the OD of the treatment and control cultures + control were determined.Achievements of the paper: In statistical analysis, it was revealed that about nanoparticles of Ag and Cu, 0.5 percent concentration of bactericidal particles are able to remove almost 100% of Klebsiella pneumoniae bacteria, Pseudomonas aeruginosa, Staphylococcus aureus and 0.1 percent concentration of each of the 3 bacteria was bacteriostatic.

Keywords:

nanoparticle, macrodilution bacteriostatic, bactericidal,

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