Cytotoxic Activities of Salvia Sahendica Extract on HeLa Cells

Abstract. The study was aimed to evaluation of the anticancer activity of the Salvia sahendica on the HeLacell line. The cytotoxicity of S. sahendicaon HeLacell was evaluated by the MTT assay.Results showed thatS. sahendicaethanolic extract has significant cytotoxiceffect on HeLacell line in concentration range between 0.156 mg/ml to 1.25 mg/ml. Maximum cytotoxiceffects were found in HeLa cells after incubation with the S. sahendica extract at 0.625 mg/ml (68.02%). Also results showed the concentrations producing 50% growth inhibition (IC50) of the S. sahendica extracts was 3.45 mg/ml. The S. sahendica extract was found dose inhibits the proliferation of cancerous HeLa cells possibly couses through rich secondary methabolits. Based on the results of this experiment extract of S. sahendica has cytotoxiceffect on HeLa cell line, and could be usefull in variety fields as the anticancer complex.

Keywords:

Salvia sahendica, ethanolic extract cytotoxic, MTT assay,

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Esmaeili, M.A., Sonboli, A., Kanani, M.R. 2009. Salvia sahendicaprevents tissue damages induced by alcohol in oxidative stressconditions: Effect on liver and kidney oxidative parameters. J MedPlants 34: 276-283. (Persian).
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Lotfipour F, Samiee M, Nazemiyeh H 2007. Evaluation of the antibacterial activity of Salvia sahendica and Phlomis caucasica. Pharmaceutical sciences, Journal of Faculty of Pharmacy, Tabriz University of Medical Sciences 1: 29-34.
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Mozafarian, V. 1996. A dictionary of Iranian plant names (Latin English Persian) Farhang Moaser Publication, Tehran, pp. 477-479.
Perry, N.S., Bollen, C., Perry, E.K., Ballard, C. 2003. Salvia for dementia therapy: review and of Pharmacology,Biochemistry and Behavior 75, 651–659. activity pilot tolerability clinical trial.
Phelan MC. 1998. Basic Techniques for Mammalian to Cell Tissue Culture. Current protocols in cell biology; 7: 1-10.
Salehi P, Sonboli A, Ebrahimi SN, Yousefzadi M 2007. Antibacterial and antioxidant activities of the essential oils and various extracts of Salvia sahendica in different henological stages. Chem. Nat. Comp. 43: 328-330.
Shaerzadeh, F., Ahmadiai, A., Esmaeili, M.A., Ansari, N., Asadi, S., Khoramian Tusi, S., Sonboli, A., Ghahremanzamane, M., Khodagholi, F. 2011b. Antioxidant and antiglycating activities of Salvia sahendica and its protective effect against oxidative stress in neuron-like PC12 cells. J. Nat. Med. 65:455-465.
Shaerzadeh, F., Alamdary, S.Z., Esmaeili, M.A., Sarvestani, N.N.,Khodagholi, F. 2011a. Neuroprotectiveeffect of salvia sahendica ismediated by restoration of mitochondrial function and inhibition ofendoplasmic reticulum stress. Neurochem Res. 36: 2216-2226.
Ulubelen, A., Oksuz, Kolak U., Johansson, C., Celik, C., Voelter, W. 2000. Antibacterial diterpenes from roots of Salvia viridis. Planta Medica 66, 458–462.
Brown, J.P. 1980. A review of the genetic effect of naturally occurring flavonoids anthraquinones and related compounds. Mutat. Res., 75:243-247.
Couladis, M., Tzakou, O., Verykokidou, E., Harvala, C. 2003. Screening of some Greek aromatic plants for anti-oxidant activity. Phytotherapy Research 17, 194−195.
Esmaeili, M.A., Sonboli, A., Kanani, M.R. 2009. Salvia sahendicaprevents tissue damages induced by alcohol in oxidative stressconditions: Effect on liver and kidney oxidative parameters. J MedPlants 34: 276-283. (Persian).
Liu, J., Shen, H.M., Ong, C.N. 2000. Salvia miltiorrhiza inhibits cell growth and induces apoptosis in human hepatoma (HepG-2) cells. Cancer Letter 153, 85–493.
Lotfipour F, Samiee M, Nazemiyeh H 2007. Evaluation of the antibacterial activity of Salvia sahendica and Phlomis caucasica. Pharmaceutical sciences, Journal of Faculty of Pharmacy, Tabriz University of Medical Sciences 1: 29-34.
Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods;65(1-2):55-63.
Mozafarian, V. 1996. A dictionary of Iranian plant names (Latin English Persian) Farhang Moaser Publication, Tehran, pp. 477-479.
Perry, N.S., Bollen, C., Perry, E.K., Ballard, C. 2003. Salvia for dementia therapy: review and of Pharmacology,Biochemistry and Behavior 75, 651–659. activity pilot tolerability clinical trial.
Phelan MC. 1998. Basic Techniques for Mammalian to Cell Tissue Culture. Current protocols in cell biology; 7: 1-10.
Salehi P, Sonboli A, Ebrahimi SN, Yousefzadi M 2007. Antibacterial and antioxidant activities of the essential oils and various extracts of Salvia sahendica in different henological stages. Chem. Nat. Comp. 43: 328-330.
Shaerzadeh, F., Ahmadiai, A., Esmaeili, M.A., Ansari, N., Asadi, S., Khoramian Tusi, S., Sonboli, A., Ghahremanzamane, M., Khodagholi, F. 2011b. Antioxidant and antiglycating activities of Salvia sahendica and its protective effect against oxidative stress in neuron-like PC12 cells. J. Nat. Med. 65:455-465.
Shaerzadeh, F., Alamdary, S.Z., Esmaeili, M.A., Sarvestani, N.N.,Khodagholi, F. 2011a. Neuroprotectiveeffect of salvia sahendica ismediated by restoration of mitochondrial function and inhibition ofendoplasmic reticulum stress. Neurochem Res. 36: 2216-2226.
Ulubelen, A., Oksuz, Kolak U., Johansson, C., Celik, C., Voelter, W. 2000. Antibacterial diterpenes from roots of Salvia viridis. Planta Medica 66, 458–462.