M. Turan ÇETİN, İbrahim ÜRÜNSAK, Abdulhakim ARISOY, Cihan ÇETİN, Taner KAFADAR, İlhan TUNCER

Tavşanlarda Overyen Otograft: Hormonal ve Morfolojik Değerlendirme

Giriş: 10 adet erişkin dişi tavşanda otograft öncesi ve sonrası ovaryumda oluşan fonksiyonel ve histolojik değişiklikler değerlendirildi. Materyal ve Metod: Preoperatif (otografttan bir ay önce) ve postoperatif (otograftan iki ay sonra) östradiol (E2) ve progesteron (P) seviyeleri ölçüldü, akabinde histolojik değerlendirmeler ışık mikroskobuyla yapıldı. Bir ay sonra, tavşanlarda ovaryum otograftı yapıldı. Sonuçlar: Ovaryum dokularının %80"inde transplante ovaryum örnekleri gözlendi. Preoperatif ve postoperatif P değerleri bazaldan geç periyoda doğru artış gösterdi ve farklar istatistiksel olarak anlamlıdır. Bazaldan geç periyoda doğru preoperatif ve postoperatif E2 değerleri arasında istatistiksel olarak anlamlı bir fark yoktur. Her üç zaman periyodunda da preoperatif P değerleri postoperatif P değerlerinden istatiktiksel olarak anlamlı şekilde yüksektir. Preoperatif E2 değeri postoperatif E2 değerine göre yüksektir fakat bu fark istatistiksel olarak anlamlı değildir. Tartışma: Eğer otograft işlemleri mikrocerrahi yöntemleri kullanılarak gerçekleştirilirse, postoperatif periyottaki ovaryum otograftları preoperatif periyotta olduğu gibi hormonal fonksiyon ve morfoloji gösterirler.

Anahtar Kelimeler:

Ovaryum otograftı, ovaryum hormonal fonksiyonu, ovaryum morfolojisi

Ovarian Autografting In Rabbits: Hormonal And Morphological Evaluations.

Purpose: The functional and histological changes in the ovarian tissue of 10 female mature rabbits were evaluated before and after ovarian autografting. Material and Methods: Preoperative (one month before the autograft) and postoperative (two months after the autograft) estradiol ( E2) and progesterone ( P) levels were measured, and histological evaluations were performed by light microscope. One month later, ovarian autografting was performed on the rabbits. Results: Ovarian tissue was observed in 80% of the transplanted ovarian samples. Both preoperative and postoperative P values increased from the basal to the late period and the differences were statistically significant. There was no significant difference between preoperative and postoperative E2 values from the basal to the late period. Preoperative P values were statistically significantly higher than postoperative P values in all three time periods. Preoperative E2 values were also higher than postoperative E2 values, but the differences were not statistically significant. Conclusion: If autografting is performed by employing microsurgical techniques, ovarian autografts will have similar postoperative hormonal function and morphology as in preoperative period.

Keywords:

ovarian autograft, ovarian hormonal function, ovarian morphology.,

PDF

___

Leporrier M, Von Theobald P,Roffe JL,Muller G :A new technique of protect ovarian function before pelvic irradiation. Cancer. 1987; 600: 2201-4.
Yamada Y: Clinical long term observations on the function of ovaries autotransplanted at radical hysterectomy on cervical carcinoma patients. Nokkaido-Igaku-Zasshi. 1985; 60: 38-47.
Muller G, Van-Theobald P, Levy G, Roffe JL, LePorrier M: First heterotopic ovarian autotransplantation in the female. J Gynecol Obstet Biol Reprod Paris. 1988; 17: 97-102.
G.Maye, J Michelot, M.Lagoutte et all: Autogreffe overienne chez lapine J Gyn Obst Biol Repr. 1979; 8:483Gunasena KT, Villines PM, Critser ES, Critser JK
Live birhts after autologous transplant of cryopreserved Mouse ovaries. Hum Reprod. 1997;12:101-6
Bedaiwy MA, Jeremias E, Gurunluoglu R, Hussein MR, Siemianow M, Biscotti C, Falcone T. Restoration of ovaran function after autotransplantation of intact frozen-tahwed sheep ovaries with microvascular anastomosis. Davies NL:Complications of ovarian aııtotransplantation in bitches.J S Afr Vet Assoc. 1989; 60:145.
Callejo J, Salvador C, Miralles A, Vilaseca S, Lailla JM, Balasch J. Long-term ovarian fuction evaluation after autografting by implantation with fresh and frozen thawed human ovarion tissue. The Journal of Clinical Endocrinology and Metabolism. 2001; 86: 4489-94
Oktay K, Buyuk E. Ovarian transplantation in humans: indications, techniques and the risk or reseeding cancer. Eur J Obstet Gynecol Reprod Biol. 2004; 113: 45-7
Baird DT, Campbell B, de Souza C, Tefler E. Longterm ovarian function in sheep after ovariectomy and autotransplantation of cryopreserved cortical strips. Eur J Obstet Gynecol Reprod Biol. 2004; 1:55-9
Bordes A,Lornage J,Demirci B,Franck M,Courbiere B,Francois Guerin J,Salle B.: Normal gestations and live births after orthotopic autograft of vitrifiedwarmed hemi-ovaries into ewes. Hum. Reprod. 2005.
Tryde Schmidt KL, Yding Andersen C, Starup J, Loft A, Byskov AG, Nyboe Andersen A.: Orthotopic autotransplantation of cryopreserved ovarian tissue to a woman cured of cancer- follicular growth, steroid production and oocyte retrieval. Reprod. Biomed Online. 2004; 8:448-53.
Donnez J, Dolmans MM, Demylle D, Jadoul P, Pirard C, Squifflet J, Martinez-Madrid B, van Langendonckt A.: Livebirth after orthotopic transplantation of cryopreserved ovarian tissue. Lancet. 2004; 364:1379-80 Yazışma Adresi / Address for Correspondence: Dr. Mehmet Turan Çetin Cukurova University Faculty of Medicine Department Obstetrics and Gynecologyı Balcalı-ADANA e-mail: info@tupbebekmerkezi.net Tlf: 0 322 2563177 geliş tarihi/received :25.12.2012 kabul tarihi/accepted:20.01.2013 .