Tayfun CEYLAN, Derya KARABULUT, Emel ÖZTÜRK, Ali Tuğrul AKİN, Emin KAYMAK, Birkan YAKAN

Sisplatin-indüklenmiş sıçan epididimis dokusunda rapamisin ve 3-metiladeninin etkilerinin histolojik olarak değerlendirilmesi

Amaç: Bu çalışmada sisplatin (Cis) doku üzerinde neden olduğu hasar üzerinde otofaji aktivatörü ve inhibitörü etkilerinin belirlenmesi amaçlanmıştır. Gereç ve Yöntem: Çalışmada her grupta 6 hayvan olacak şekilde 24 adet Wistar albino cinsi erkek sıçan kullanıldı. Gruplar; Kontrol grubu, Sisplatin grubu (Cis) (8 mg/kg), Rapamisin (Rapa) grubu (2 mg/kg), 3-metiladenin grubu (3-MA) (15 mg/kg). Rapa ve 3-MA intraperitoneal 15 gün boyunca, Cis tek doz deneyin 7. Günü uygulandı. Deney sonunda epididimisler alındı. Histolojik teknikler sonrası alınan kesitlere Hematoksilen-Eozin ve Heat-shock-protein70 (HSP70) immunohistokimyası uygulandı. Bulgular: Cis kesitlerinde tübül bazal membranında dağılma, tübül içinde vakuolizasyon gözlendi. Ayrıca bazı epitel hücrelerinin daha eozinofilik olduğu gözlendi. Rapa ve 3-MA ait doku kesitleri epitelizasyon, tübül bazal membranı olarak daha düzenli bir görünüme sahipti. HSP70 immunreaktivitesi tüm grupların tübüler arası bağ dokusunda gözlendi. Sonuç: Semen kalitesi ve spermatozoanın potansiyelliğinin korunması için organın Cis gibi ajanlardan etkilendiği sonucuna varılmıştır. Rapa, Cis toksisitesine karşı epididimis üzerinde 3-MA’den daha etkili olabilir.

Anahtar Kelimeler:

Epididimis, Otofaji, Rapamisin, Sisplatin, 3-Metiladenin

Histological evaluation of the effects of rapamycin and 3-methyladenine on cisplatin-induced epididymal injury in rats

Purpose: In this study, we aimed to determine the effects of autophagy inhibitor and activator on Cisplatin (Cis)-induced tissue damage. Materials and Methods: A total of 24 male Wistar albino rats were divided into 4 groups including 6 rats per group in this study. Groups are as follows; Control, Cisplatin (Cis) (8 mg/kg), Rapamycin (Rapa) (2 mg/kg), 3-methyladenine (3-MA) (15 mg/kg). Rapa and 3-MA were given intraperitoneally for 15 days. Cis was administered as a single dose on the 7th day of the experimental period. At the end of the experimental procedure, epididymis tissues were extracted. Hematoxylin and eosin staining and Heat shock protein-70 (HSP70) immunohistochemistry were applied to the sections taken after histological techniques. Results: Dispersion in the tubule basement membrane and vacuolization in the tubule was observed in the Cis group. It was also observed that some epithelial cells were more eosinophilic in the Cis group. Tissue sections of Rapa and 3-MA had a more regular appearance in terms of epithelization and tubule basement membrane. HSP70 immunoreactivity was observed in the intertubuler connective tissue of all groups. Conclusion: The epididymis was affected by agents such as Cis in terms of the protection of semen quality and potency of spermatozoa. Rapa may be more effective than 3-MA in the epididymis against Cis toxicity.

Keywords:

Autophagy, Cisplatin, Epididymis, Rapamycin, 3-Methyladenin,

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