Mesut METE, Kamil VURAL, Enis CEZAYİRLİ, Tuncay VAROL, Mehmet İbrahim TUĞLU

Sıçan omuriliğinde nosiseptif uyaranlardan sonra ağrı düzenlemesinde nitrik oksidin rolü

Amaç: C-fos indüksiyonunun, periferik inflamasyona karşı spinal kordda meydana gelen nöron yanıtının düzenlenmesinde önemli bir rol oynamaktadır. Sinir dokusunda NADPH-diaphorase (NADPH-d) reaksiyonu nitrik oksit sentaz (NOS) aktivitesinin iyi bir göstergesidir.Gereç ve Yöntem: On üç erkek Wistar albino sıçan kullanıldı. Sıçanlar 3 gruba ayrıldı. Grup 1: ağrı grubu (n = 5). Grup 2: şam grup (n = 5). Grup 3: kontrol grubu (n = 3). Grup 1'de, 50 μl% 30 formalin solüsyonu, sıçan sağ ayağın dorsal yüzeyi üzerine tek taraflı subkutanöz enjekte edildi. Grup 2'de PBS, sıçan sağ ayağın dorsal yüzeyinde tek taraflı subkutanöz enjekte edildi. Grup 3'de ise tedavi verilmedi. Her grupta lumbar spinal cord (SC) örnekleri alındı. Numuneler c-fos immünohistokimyası, NADPH-d histokimyası ve c-fos / NADPH-d ikili etiketleme yöntemleriyle histokimyasal olarak boyandı ve ışık mikroskopu altında değerlendirildi.Bulgular: Spinal kord kesitlerinde, hücrelerin c-fos, NADPH-d ile boyandığını ve her ikisinin de nosiseptif uyaranlarla belirginleştiğini saptadık. Ağrı grubunda, şam grubuna göre c-fos (+) (CF), NADPH-d (+) (NA) ve çift-etiketli (CN) hücre sayısında belirgin artış saptandı. Grup 1'de NADPH, spinal kord lomber segment kesitlerinin her iki yanındaki I-IV. laminalarında, aynı tarafta ise VIII, IX ve X laminalarında anlamlı olarak artmış halde bulundu. Sonuç: Bulgular, NO'nun spinal kordda, formaline bağlı FOS-ir ile ilişkili olduğunu, ağrının düzenlenmesinde önemli bir rol oynayabileceğini ve nosiseptif fonksiyonun artmış nitrik oksit sentaz (+) nöronlarla ilişkili olduğunu göstermektedir.

Anahtar Kelimeler:

Nitrik oksit; Ağrı; Spinal kord

Role of nitric oxide in the regulation of pain after nociceptive stimuli in rat spinal cord

Purpose: In this study, we examined the responses of the neurons in the lumbar segments of the spinal cord to nociceptive stimuli induced by formalin using c-fos immunohistochemistry and NADPH-d histochemistryMaterials and Methods: Thirteen male, Wistar albino rats were used. The rats were divided into 3 groups. Group 1: pain group (n=5). Group 2: sham group (n=5). Group 3: control group (n=3). In group 1, 50 µl of 30% formalin solution was injected subcutaneously unilaterally on the dorsal surface of the right foot. In group 2, phosphate buffered saline was injected subcutaneously unilaterally on the dorsal surface of the right foot. In group 3, no treatment was given. For each group lumbar spinal cord (SC) samples were taken. Samples were stained with c-fos and NADPH-d histochemistry and c-fos/NADPH-d double-labeling methods and evaluated under light microscope.Results: We noted that cells on spinal cord sections stained with c-fos, NADPH-d and both became prominent with nociceptive stimuli. There were significant increases in the numbers of c-fos (+), NADPH-d (+) and double-labeled cells in pain group comparison to sham group. In group 1, NADPH was significantly increased in the Laminae I-IV of the lumbar segments of the SC on both sides and laminae VIII, IX and X on the same side. Conclusion: The findings suggest that NO is associated with formalin-induced FOS-ir in the spinal cord, that it may play an important role in the regulation of pain and that the nociceptive function is related to increased nitric oxide synthase (+) neurons in the spinal cord.

Keywords:

Nitric oxide, Pain Spinal Cord,

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