Remzi GOKDENİZ, Ali OVAYOLU, Robert GARFİELD

Progesteronun insan myometrium kontraksiyonlarını membran reseptörleri aktivasyonu ile inhibisyonu

Geçmiş: Myometriyal inhibisyon mekanizmaları hala araştırılmaktadır. Amaç: Uterin kontraksiyonlar üzerine progesteron (P4) inhibisyon mekanizmalarını değerlendirmektir. Metot: Prospektif çalışma St. Joseph"s Hospital ve Maricopa Hospital, Phoenix, AZ and research center in Arizona, ABD\'deyapılmıştır. Çalışma 2010-2011 yılları arasında sezaryan ile doğum yapmış kadınlarda yapıldı. Termde 24 kadından toplanan uterin dokular çeşitli ajanlara maruz bırakıldı. Kontraktilite, ajanların öncesi ve sonrasında karşılaştırıldı ve kaydedildi. Dokular progesteron membran reseptörüne (mPR), düşük affinite gösteren sadece bir progestinle (R5020) veya non-seks stereoid (kolesterol) ile tedavi edildi. Diğer dokular ise adenilatsiklaz (SQ 22536), guanilatsiklaz (ODQ), fosfodiesteraz (rolipram), nitrikoksit (NO) sentaz (L-NAME) inhibitörleri veya bir nükleer P4 reseptörantagonisti (mifepristone, MIF) uygulandıktan sonra, P4 ile tedavi edildi. Veriler ANOVA ile analiz edildi. Sonuçlar: P4 uterin kontraksiyonları inhibe etti (P0.05). P4 inhibisyonu MIF, SQ, ODQ, rolipram veya L-NAME ile etkilenmemiştir (P>0.05). Tartışma :P4 cAMP, cGMPveya NO aracılığı ile değil; nongenomik mPR aktivasyonu ile myometrial kontraksiyonlari hızlıca inhibe etmektedir.

Anahtar Kelimeler:

Siklik adenozin monofosfat; myometriyum; erken doğum; progesteron reseptörleri; uterin kontraktilite

Progesterone Inhibits Human Myometrial Contractions by Action on Membrane Receptors

Background: The mechanisms for myometrial inhibition are still being investigated Aim: To examine mechanisms of progesterone (P4) inhibition of uterine contractility. Methods: Prospective study Tertiary care center at St. Joseph"s Hospital and at Maricopa Hospital, Phoenix, AZ and research center in Arizona, USA. During 2010-2011, 24 women given birth by cesarean section. Uterine tissues from women (n=24) at term were suspended in organ chambers and exposed to various agents. Contractility was registered and compared before and after addition of agents. Tissues were treated with P4 alone, a progestin (R5020) with low affinity to the progesterone membrane receptor (mPR), or a non-sex steroid (cholesterol). Other tissues were pretreated with inhibitors of adenylate cyclase (SQ 22536), phosphodiesterase (rolipram), nitric oxide (NO) synthases (L-NAME) or a nuclear P4 receptor antagonist (mifepristone, MIF), followed by P4. Data were analyzed by ANOVA. Results: P4 (P0.05) inhibitory effects. P4 inhibition is not blocked by MIF, SQ, ODQ, rolipram or L-NAME (P>0.05). Conclusions: P4 rapidly inhibits myometrial contractility by nongenomic mechanisms through action on mPR but not via cAMP, cGMP, or NO

Keywords:

Cyclic adenosine monophosphate; myometrium; preterm labor; progesterone receptors; uterine contractility,

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Yazışma Adresi / Address for Correspondence: Dr. Ali Ovayolu İnönü University Medical Faculty Department of Obstetrics and Gynecology MALATYA e-mail: drovayolu@yahoo.com geliş tarihi/received :22.08.2012 kabul tarihi/accepted:27.10.2012