Emine KILIÇ BAĞIR, Arbil AÇIKALIN, Melek ERGİN, Gülay SEZGİN, Serhan KÜPELİ, Gülşah SEYDAOĞLU

Pediatrik lenfomalarda histopatolojik subtip ve Ebstein-Barr virus ilişkisi: immünohistokimyasal ve in situ hibridizasyon çalışması

Amaç: Bu çalışmanın amacı bölgemizdeki pediatrik lenfomaların histopatolojik alt tiplerinin oranını ve bunların Ebstein-Barr virüs enfeksiyonu ile olan ilişkisini incelemektir.Gereç ve Yöntem: Hodgkin lenfoma tanısı alan 36 olgu ve non-hodgkin lenfoma tanısı alan 51 olgu retrospektif arşiv taraması yapılarak çalışmaya dahil edildi. Olgulara immünohistokimyasal olarak Ebstein-Barr virüs latent membran proteini-1 ve in-situ hibridizasyon uygulandı.Bulgular: Hodgkin lenfoma olgularında; en sık rastlanan histopatolojik alt tipler sırası ile mikst sellüler klasik hodgkin lenfoma (%55,6) idi. Non-Hodgkin lenfoma olgularında en sık rastlanan histopatolojik alt tip Burkitt lenfoma (%51) idi. Ebstein-Barr virüs latent membran proteini-1 ile 29 hodgkin lenfoma olgusunda pozitif iken non-hodgkin lenfoma olgularında immünohistokimya ile pozitif boyanma saptanmadı. Hodgkin lenfoma olgularında in situ hibridizasyon ile boyanma oranı % 83.,3 iken non-Hodgkin lenfoma olgularında bu oran % 27.5 idi.. Her iki boyama yöntemi için gruplar arasındaki fark istatistiksel olarak anlamlıydı.Sonuç: Bu çalışmada, olgularımızın histopatolojik dağılımlarının gelişmekte olan ülkelerdeki pediatrik lenfoma olguları ile benzer olduğunu göstermektedir. Aynı zamanda in situ hibridizasyon’da Ebstein-Barr virüs pozitifliği daha fazla olması nedeniyle in situ hibridizasyonun ebstein barr virüs tespitinde immünohistokimyadan daha spesifik olduğunu düşünmekteyiz.

Anahtar Kelimeler:

Ebstein Barr virüs, Hodgkin lenfoma, İmmünohistokimya, İn situ hibridizasyon, Non-Hodgkin lenfoma, pediatrik

Histopathological subtypes of pediatric lymphomas and relation to Ebstein-Barr virus: an immunohistochemical and in situ hybridization study

Aim: Lymphomas are the second most common malignant disease after leukemia and brain tumors in developing contries. Ebstein-Barr virus has a initiator role in the development of lymphoma, but it is known that socioeconomic conditions, genetic features and the immune supressive factors is also important. We aimed to explore the histopathologic subtypes of pediatric lymphomas and their relation with Ebstein-Barr virüs infection in our region.Material and Methods: In this retrospective study, 87 children including 36 cases with hodgkin lymphoma and 51 cases with non-hodgkin lymphoma were included in the study. The pathologic slides were used to investigate immunohistochemical staining with Ebstein-Barr virüs latent membrane protein-1 and in-situ hybridization. Results: The most common histopathological subtype in hodgkin lymphoma cases were mixed cellular classical Hodgkin lymphoma (55.6%) followed by nodular sclerosis classical hodgkin lymphoma (16.6%). The most common histopathological subtype in non-Hodgkin lymphoma cases was Burkitt lymphoma (51.0%) followed by precursor T-cell lymphoblastic leukemia/lymphoma (13.7%). Ebstein-Barr virüs latent membrane protein-1 was positive in 29 Hodgkin lymphoma. All non-Hodgkin lymphoma cases were stained negative with ımmunohistochemical. The positive staining rate of Hodgkin lymphoma cases with in-situ hybridization was 83.3% and this rate was 27.5% in non-Hodgkin lymphoma cases. The differences between groups for both staining methods were statistically significant (p<0.001) Conclusion: The results of this study show that the distribution of histopatholojik subtypes of pediatric lymphomas similar to in developing countries. And we have observed that in-situ hybridization is more specific than immunohistochemistry because Ebstein-Barr virus positivity is more detected in in-situ hybridization.

Keywords:

Ebstein-Barr virüs, Hodgkin Lymphoma; Immunohistochemistry; In situ hybridization, pediatric, Non-Hodgkin Lymphoma,

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