Anggun İndah BUDİNİNGRUM, Achmad ROFİ'İ, Suharjono SUHARJONO, Fatchiyah FATCHİYAH

LMP-1 ve Nazofaringial Karsinoma

Amaç: Bu çalışmanın amacı nazofaringial karsinomun litik fazı üzerine etkili olduğu düşünülen LMP-1, ile H2O2 , nitrozamin ve nikotinin gibi dış faktörler arasındaki etkileşimi belirlemektir. Materyal ve Metod: insan LMP-1 (GI: 343177335) aminoasid sekansı ile nikotin (ID: 89594), nitrozamin (ID: 37183) ve H2O2 (ID: 37183) nin yapısal içeriği The National center of Biotechnology Information'a ait veri tabanına göre açıklanmıştır. LMP-1 in 3 boyutlu yapısı SWISS-MODEL web ağı kullanarak tasarlandı. Hex 6,12 hedef moleküle öncülük etmesi için kullanıldı. LigPlus+1.4.4 software ile ligand detayları tarandı. Moleküler grafikler ve analizler PyMOL ile yapıldı. Bulgular: LMP-1 in H2O2 , nitrozamin ve nikotin ile etkileşimi vardır. LMP-1 in H2O2 etkileşimi için harcadığı enerji (-67,79 kj/mol), nikotin (-149,43 kj/mol) ve nitrozamin (-82,93 kj/mol) için kullanılandan daha düşüktür. LMP-1 ile H2O2 arasındaki etkileşimin türü Hidrojen bağı iken LMP-1 ile nitrozamin ve nikotin arasındaki etkileşim hidrofobik bağdır. Sonuç: LMP-1 H2O2 ile nitrozamin ve nikotine göre daha güçlü bağ kurar. Dış etken olan H2O2 nin LMP-1 i etkileyerek NPC li hastalarda litik enfeksiyon fazına geçişe neden olduğu tahmin edilmektedir.

Anahtar Kelimeler:

H2O2 , LMP-1, nazofaringial karsinom, nikotin, nitrozamin.

LMP-1 and Nasopharyngeal Carcinoma (NPC)

Purpose: This study aims to determine the interaction of LMP-1 with H2O2 , nitrosamine, and nicotine to predict the external factor that affected LMP-1, which activates lytic phase on nasopharyngeal carcinoma (NPC). Materials and Methods: The amino acid sequence of human LMP1 (GI: 343177335) and compound structure of H2O2 (ID:784), nicotine (ID: 89594), and nitrosamine (ID: 37183) was obtained from database The National center of Biotechnology Information. The 3-dimension modeling structure of LMP-1 predicted using SWISS-MODEL web server. Hex 6.12 was used for the purpose of docking of the lead with the target molecule. Ligand details interaction then detected by LigPlus+ v.1.4.4 software. Molecular graphics and analysis were performed with PyMOL. Results: LMP-1 has interaction with H2O2 , nitrosamine, and nicotine. Total energy that use of LMP-1 to interaction with H2O2 (-67,79 kJ/mol) is lowest than interaction with nicotine (-149,43 kJ/mol) and nitrosamine (-82,93 kJ/mol). The kind of interaction between LMP-1 and H2O2 is hydrogen bond whereas interaction of LMP-1 between nicotine and nitrosamine are hydrophobic bond. Conclusions:LMP-1 has strongest interaction with H2O2 than nicotine and nitrosamine. This is predicted that H2O2 is external factor that affect LMP-1 switching to lytic infection in NPC patients.

Keywords:

H2O2, LMP-1; nasopharyngeal carcinoma, nicotine, nitrosamine,

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