Syiska Atik MARYANTİ, Siti SUCİATİ, Endang Sri WAHYUNİ, Sanarto SANTOSO, İ Wayan Arsana WİYASA

Rodamin B, Oksidatif Stress Aracılığı ile Ovarian Toksisitesi

Amaç: Çalışmanın amacı, rodamin B"ye maruziyetin, ovarian oksidatif stress, ovarian folikül, 17β-Estradiol hormonu ve endomerium kalınlığı üzerindeki etkisini araştırmak. Materyal Metod: Toplam 28 dişi sıçan kontrol grubu ile birlikte 4 gruba ayrılmıştır. Gruplar 4.5, 9 ve 18 miligram/200 gram vücut ağırlığı olacak şekilde rodamin B ile muamele edilmişir.Rodamin B, 36 gün boyunca probla birlikte oral olarak uygulanmıştır. MDA seviyelerinin analizi spektrofotometrik olarak yapılmıştır. Ovarian folikül sayısı ve endometrium kalınlığı analizleri histopatolojik olarak hematoksilin eozin boyama ile yapılmıştır. 17β-Estradiol seviyesi ELISA ile ölçülmüştür. Bulgular: Dişi sıçanlara farklı dozlarda Rodamin B uygulanması, ovarian MDA seviyelerini kontrollere gore anlamlı şekilde arttırmıştır (P < 0.05). Dişi sıçanlarda rodamin B"nin 2. ve 3. dozları primer, sekonder ve De Graaf folikül sayısını kontrollerle karşılaştırıldığında anlamlı olarak azaltmıştır (P < 0.05). Dişi sıçanlarda rodamin B"nin 2. ve 3. dozlarının uygulanması 17β-estradiol seviyesini kontrollerle karşılaştırıldığında anlamlı olarak azaltmıştır (P < 0.05). Rodamin B uygulanması endometrium kalınlığını kontrollerle karşılaştırıldığında anlamlı olarak azaltmıştır (P < 0.05). Sonuç: Rodamin B uygulamasının, oksidatif stress aracılığı ile ovarian toksisitesini tetiklediği, folikül sayısını azalttığı ve, 17β-Estradiol seviyesini azaltalarak endometriyum kalınlığını düşürdüğü sonucuna varılmıştır.

Anahtar Kelimeler:

Boya, Toksisite, ovarian, endometriyum, oksidatif stres

Rhodamine B triggers ovarian toxicity through oxidative stress, decreases in the number of follicles, 17B-estradiol level, and thickness of endometrium

Objective: The purpose of this study was to analyze the effects of exposure to rhodamine B on ovarian oxidative stress, ovarian follicles, hormone 17-estradiol and thickness of endometrium. Methods: A total of 28 female rats were divided into four groups consisting of control; groups treated with rhodamine B at doses of 4.5; 9, and 18 milligram/200 gram body weight. Rhodamine B was administered orally for 36 days with the probe. Analysis of MDA level was done spectrophotometrically. Analysis of the number of ovarian follicles and thickness of endometrium was done histopathologically by hematoxylin eosin staining. Analysis of 17-estradiol level was done by ELISA. Results: Rhodamine B administered in different doses in female rats can increase ovarian MDA levels significantly than the control (P 0.05). Administration of rhodamine B of the second and third doses in female rats can reduce the number of primary, secondary, and De Graaf follicles significantly compared to the control (P 0.05). Administration of rhodamine B of the second and third doses in female rats can reduce 17-estradiol level significantly compared to the control (P 0.05). The administration of rhodamine B could reduce thickness of endometrium significantly compared to the control (P 0.05). Conclusion: It was concluded that administration of rhodamine B triggered ovarian toxicity through oxidative stress, a decrease in the number of follicles, and decreased level of 17-estradiol which ultimately lowered the thickness of endometrium.

Keywords:

dye; toxicity; ovarium; endometrium; oxidative stress.,

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Yazışma Adresi / Address for Correspondence: Dr. Syiska Atik Maryanti Brawijaya University Faculty of Medicine Midwifery Master Study Programme, Jl. Veteran, Malang, East Java, INDONESIA Email: syiska_hermawan@yahoo.com G eliş tarihi/Received on: 30.01.2014

Kabul tarihi/Accepted on:28.02.2014