Dhay Ali AZEEZ, Duygu FINDIK, Hatice TÜRK DAĞI, Uğur ARSLAN

Konya, Türkiye’de Escherichia coli klinik izolatlarında plazmid aracılı fluorokinolon direnci

Amaç: Kinolonlara karşı direnç gelişimi, genellikle kromozomlarla kodlanan topoizomeraz ve efflux pump genlerindeki mutasyonlara, porin kaybına ve plasmid aracılı genlerin transferine bağlı olarak gelişmektedir. Bu çalışmanın amacı, Selçuk Üniversitesi, Konya/Türkiye’de Escherichia coli klinik izolatlarında plazmid aracılı kinolon direnç genlerinin (qnrA, qnrB, qnrC, qnrS, qepA, and aac(6’)-1b-cr) varlığını araştırmaktır.Gereç ve Yöntem: Bu çalışmada, qnrA, qnB, qnrC, qnrS, qepA, and aac (6’)-1b-cr genleri kinolon dirençli 115 E. coli suşunda polimeraz zincir reaksiyonu (PZR) ile araştırıldı. Tüm aac (6’)-1b-cr varyantını tanımlamak için aac (6’)-1b pozitif amplikonlar BseGI restriksiyon enzimiyle kesilerek araştırıldı. Bulgular: 115 kinolon dirençli E.coli suşundan üçünde (% 2.6) qnrB, dokuzunda (% 7.8) qnrS ve ellisinde (% 43.5) aac (6’)-1b-cr genleri pozitif bulundu. Suşların hiçbirinde qnrA, qnrC ve qepA genleri tespit edilmedi.Sonuç: Türkiye Konya’da E. coli klinik suşlarında kinolon direncinden, aac (6 ‘)-1b-cr geninin sorumlu olduğu belirlendi. qnrB ve qnrS gen sıklığı düşük olarak tespit edildi ve qnrA, qnrC ve qepA genleri tespit edilmedi. Kinolon direnç genlerinin sürveyansı önemlidir, özellikle plazmid aracılı olanlar tüm dünyaya hızla yayılmaktadır.

Anahtar Kelimeler:

Escherichia coli, plazmid aracılı florokinolon direnci, qnr, qepA, aac (6’)-1b-cr

Plasmid-mediated fluoroquinolone resistance in clinical isolates of Escherichia coli in Konya, Turkey

Purpose: Resistance to quinolones usually results from mutations in the topoisomerase genes encoded chromosomally and also the expression of efflux pumps, loss of porines and the transfer of plasmid-mediated genes. The aim of this study was to investigate the presence of plasmid-mediated quinolones resistance genes (qnrA, qnrB, qnrC, qnrS, qepA, and aac(6’)-1b-cr) in clinical isolates of Esherichia coli from Selcuk University, Konya, Turkey.Materials and Methods: In this study 115 quinolone-resistant isolates were screened for qnrA, qnrB, qnrC, qnrS, qepA, and aac(6’)-1b-cr genes by polymerase chain reaction (PCR). All aac(6’)-1b positive amplicons were analyzed by digestion with BseGI restriction enzyme to identify aac(6’)-1b-cr variant.Results: Of the 115 quinolone-resistant E.coli strains, three (2.6%) carried qnrB, nine (7.8%) carried qnrS and 50 (43.5%) carried aac(6’)-1b-cr genes. None of them harboured qnrA, qnrC and qepA genes.Conclusion: We determined that aac(6')-1b-cr gene was responsible for most of the quinolone-resistant E. coli strains from Konya, Turkey. The prevalence of qnrB and qnrS genes was low and qnrA, qnrC and qepA genes were not detected. The surveillance of quinolone resistance genes is important, especially plasmid mediated ones are rapidly spreading all over the world.

Keywords:

Escherichia coli, plasmid-mediated fluoroquinolone resistance; qnr; qepA, aac(6’)-1b-cr,

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