Klorzoksazon’un antibakteriyel etkisinin belirlenmesi

Amaç: Antibiyotik olmayan ilaçların bazıları, rutinkullanımlarında bakteri gelişimine karşı inhibisyon etkisağlayabilirler. Klorzoksazon, kas spazmı ve bununlabirlikte ortaya çıkan ağrıyı tedavi etmek için kullanılan kasgevşetici ilaçtır. Bu çalışmanın amacı Klorzoksazon ‘unantibakteriyel etkilerinin MİK (minimum inhibitörkonsantrasyon), MBK (minimum bakterisidkonsantrasyon) ve disk difüzyon metodu ileincelenmesidir.Gereç ve Yöntem: Klorzoksazon’a karşı MİK ve MBKdeğerlerin belirlenmesi 2 farklı bakteri suşunda (gramnegatif bakteri suşu olarak Pseudomonas aeruginosa vegram pozitif bakteri olarak Bacillus subtilis) seyreltmesiyöntemine göre belirlenmiştir. Disk difüzyon testi ise üçfarklı konsantrasyon (25, 50 ve 100 μg)’da yapılmıştır.Bulgular: Klorzoksazon’un Pseudomonas aeruginosa'yakarşı MİK değeri 800 mg mL-1, MBK değerleri ise 2.56 mgmL-1 ve Klorzoksazon 'un Bacillus subtilis'e karşı MİKdeğerleri 0.400 mg mL-1, MBK değeri ise 1.92 mg mL-1olarak belirlenmiştir. Disk difüzyon deneyinden elde edilensonuçlar Klorzoksazon’un Pseudomonas aeruginosa veBacillus subtilis üzerinde antibakteriyel etkisinin olmadığısonuçlanmıştır.Sonuç: Klorzoksazon’un antibakteriyel aktivitesininolmamasına rağmen Klorzoksazon’un muhtemel riskinibelirlemek için başka çalışmalara ihtiyaç vardır.

Investigation of antibacterial effects of chlorzoxazone

Purpose: Some of non-antibiotic drugs may provide inhibition against bacterial growth in their routine use. Chlorzoxazone is a muscle relaxant used to treat muscle spasm and the resulting pain or discomfort. This study was aimed to the investigation of antibacterial effects of Chlorzoxazone using MIC (Minimum inhibitory concentration), MBC (minimum bactericidal concentration) and disk diffusion method. Materials and Methods: The MIC and MBC was determined by dilution assay using different concentrations of Chlorzoxazone against 2 bacterial strain (Pseudomonas aeruginosa as a gram negative bacterial strain and Bacillus subtilis as a gram positive bacterial strain). Disk diffusion assay were prepared at different doses (25, 50 and 100 µg). Results: MIC values of Chlorzoxazone against Pseudomonas aeruginosa was 0.800 mg ml-1, and MBC values was 2.56 mg ml-1 and MIC values of Chlorzoxazone against Bacillus subtilis was 0.400 mg ml1, and MBC values was 1.92 mg ml-1. The results obtained from disk diffusion assay supported that the Chlorzoxazone has not antibacterial effect on Pseudomonas aeruginosa and Bacillus subtilis. Conclusion: Although antibacterial activity of Chlorzoxazone was not found but another studies are needed to determine the probable risk of Chlorzoxazone.

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