Gastrik kanserde FABP4 gen ekspresyon analizi

Amaç: Gastrik kanser birçok ülkede yüksek oran ve mortalite oranına sahiptir ve günümüzde halen en sık görülen ölümcül hastalıklarından biridir. Bu çalışmada, gastrik kanseri teşhisini için moleküler tekniklerle FABP4 geninin mRNA ekspresyon analizini içeren yeni markırlar belirlemeyi amaçladık. Yağ asidi bağlayıcı protein 4 (FABP4) geni adipositlerde bulunan yağ asidi bağlayıcı proteini kodlar. FABP4 tarafından kodlanan protein, uzun zincirli yağ asitleri ve diğer hidrofobik ligandlara bağlanan küçük, yüksek düzeyde korunmuş, sitoplazmik bir protein ailesidir. FABP’ün yağ asidi alımı, taşınması ve metabolizmayı içeren görevleri olduğu düşünülmektedir.Gereç ve Yöntem: Total RNA, 47 gastrik kanserinin normal dokularından ve eşleştirilmiş tümörden çıkarılmıştır. FABP4 mRNA ekspresyon seviyesi yarı-kantitatif reverse transkripsiyon polimeraz zincir reaksiyonu (RT-PCR) kullanılarak ölçülmüştür.Bulgular: FABP4’ün mRNA ekspresyon seviyesi önemli ölçüde azalmıştır (down regüle olmuştur).Sonuç: FABP4 geninin down-regülasyonunun, gastrik kanserinin başlangıç aşamalarında meydana geldiği görünmektedir. İmmünohistokimya ve epigenetik teknikler gibi ileri analizler, FABP4 geni ve gastrik kanser arasındaki ilişkiyi doğrulamak için gereklidir.

Anahtar Kelimeler:

Gastrik kanser, FABP4, ekspresyon analizi, yarı-kantitatif RT-PCR

Gene expression analysis of FABP4 in gastric cancer

Purpose: Gastric cancer has high incidence and mortality rate in several countries and is still one of the most frequent and lethal disease. In this study, we aimed to determine diagnostic markers in gastric cancer by molecular techniques; include mRNA expression analysis of FABP4 gene. Fatty acid binding protein 4 (FABP4) gene encodes the fatty acid binding protein found in adipocytes. The protein encoded by FABP4 are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. Material and Methods: Total RNA were extracted from paired tumor and normal tissues of 47 gastric cancer. The mRNA expression level of FABP4 was measured employing semi- quantitative reverse transcription- polymerase chain reaction (RT- PCR). Results: The mRNA expression level of FABP4 was significantly decreased (down- regulated). Conclusion: Down-regulation of FABP4 gene seems to occur at the initial steps of gastric cancer development. In order to confirm the relationship between the gastric tumor and FABP4 gene, further analysis like immunohistochemistry and epigenetc techniques are necessary.

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