Arina Novilla, As&apos;ari Nawawi, Ganthina Sugihartina, Wahyu Widowati

Endonezya"da ki Propolis Ekstratlarının Metisiline Dirençli Staphylococcus aureus\'lara Karşı Antibakteriyal ve Antioksidatif Aktiviteleri

Amaç: Bu çalışma Endonezyada ki propolis ekstratlarının, metisiline Dirençli Staphylococcus aureus" lara (MRSA) yönelik antibakteriyal ve antioksidatif aktivitelerini tespit etmeyi amaçlamaktadır. Materyal ve Metod: Antioksidan aktivitesi DPPH radikali ve H2O2 giderme metodu kullanılarak değerlendirilmiştir. MRSA'ya yönelik antibakteriyel aktivite ise Kirby Bauer agar diffusion yöntemi kullanılarak test edilmiştir. Bulgular: Konsantrasyon %100-%15.525 iken Vitamin C'nin DPPH radikalini giderme aktivitesi; etanolün, n-hexane ve etil asetat ekstratlarının aktivitesinden daha yüksektir. Konsantrasyon %7.8125 iken etanol ekstraktının DPPH radikalini giderme aktivitesi %37.170, n-hexane'ın aktivitesi %38,310, etil asetatın aktivitesi %36.807"dir. H2O2 giderme aktivitesi üç propolis ekstratında, Vitamin C ye nazaran daha yüksektir (%86.642), öyle ki 7,813 ug / mL 'lik bir n-hexane fraksiyonunun giderme aktivitesi %94.925, etanol ekstraktının ki % 94.617 ve etil asetat fraksiyonunun ki %87.608"dir. Etanol ekstraktının oranı 500 ug/mL olan propolis, maksimum total fenol seviyesine sahiptir (%59.1250). Etanol ve etil aseteat fraksiyonları üç tip bakterinin hepsinide inhibe edebilirler. Etanol fraksiyonu S. aureus ve B. subtilis' in sadece gelişmesini inhibe edebilir. N-hexane üç tip bakterinin hiçbirini inhibe edemez. Etanol ve etil asetat için minimum inhibitor konsantrasyonu (MIC) değerleri, 2 mm lik inhibisyon bölgesinde %4 (40 mg/mL) olarak tespit edilmiştir. Sonuç: Propolis ekstraktı fenolleri içerir, serbest radikalleri ve H2O2 yi giderebilir, böylece oksidatif stresi engeller. Propolis içinde ki etil asetat fraksiyonu ise etanol ve n-hexane fraksiyonlarından daha fazla olarak antibakteriyel aktiviteye sahiptir. Buna ek olarak propolis ekstraktı, etanol ve etil asetat fraksiyonları MRSA ya karşı antibakteriyeldir.

Anahtar Kelimeler:

serbest radikaller, oksidan, Gram pozitif bakteriler, Gram negatif bakteriler, spor üreten bakteriler.

Antioxidative and antibacterial activities of Indonesian Propolis Extracts Against Methicillin-Resistant Staphylococcus aureus (MRSA) in Vitro

This study aims at determining the antioxidant and antibacterial activities of Indonesian propolis extracts against Methicillin-Resistant Staphylococcus aureus (MRSA). The antioxidant activity was assessed using DPPH radical and H2O2 scavenging method. Antibacterial activity of propolis extracts against MRSA (Methicillin-Resistant Staphylococcus aureus) was tested using the Kirby Bauer agar diffusion method. At concentration of 100%-15.525%, the DPPH radical scavenging activity of vitamin C is higher than that of the ethanol, n-hexane, and ethyl acetate extracts. At a concentration of 7.8125%, the DPPH radical scavenging activity of the ethanol extract is 37.170 %, n-hexane at 38,310% and ethyl acetate at 36.807%. H2O2 scavenging activities of the three propolis extracts are higher than that of vitamin C (86.642%), in which the scavenging activity for a fraction of 7.813 ug/mL n-hexane is 94.925 %, ethanol extract at 94.617 % and ethyl acetate fraction at 87.608%. Ethanol extract of 500 ug/mL propolis has the highest total phenol level at 59.1250%. Ethanol and ethyl acetate fractions are capable of inhibiting all three types of bacteria. Ethanol fraction is only able to inhibit the growth of S. aureus and B.subtilis. N-hexane fraction cannot inhibit all three types of bacteria. Minimum inhibitory concentration (MIC) values for ethanol and ethyl acetate extracts occur at a concentration of 4% (40 mg/mL) with 2-mm inhibition zone. Propolis extract contains phenols and can scavenge free radicals and H2O2 and thus potentially inhibit oxidative stress. Ethyl acetate fraction of propolis has antibacterial activity which is greater than the ethanol and n-hexane fractions. In addition, propolis extract, ethanol fraction and ethyl acetate fraction are antibacterial against MRSA.

Keywords:

free radicals, oxidants, Gram-positive bacteria, Gram-negative bacteria, spore-producing bacteria.,

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Yazışma Adresi / Address for Correspondence: Dr. Wahyu Widowati Faculty of Medicine Maranatha Christian University Jl. Surya Sumantri 65 Bandung Jawa Barat Province, INDONESIA E-mail : Wahyu_w60@yahoo.com geliş tarihi/received :16.12.2013 kabul tarihi/accepted:09.01.2014